Oh Kelly H, Kim Hongkyun
Department of Cell Biology and Anatomy, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, USA.
Bio Protoc. 2017 Jul 20;7(14). doi: 10.21769/BioProtoc.2400.
Aldicarb treatment causes an accumulation of acetylcholine in the synaptic cleft of the neuromuscular junction, resulting in sustained muscle activation and eventually paralysis. Aldicarb-induced paralysis assay is an easy and fast method to determine whether synaptic transmission of a mutant of interest is altered. This assay is based on the correlation of the rate of neurotransmitter release with the rate of paralysis. In this protocol, we describe a method for simultaneously assessing the aldicarb sensitivity of animals with different genotypes.
涕灭威处理会导致神经肌肉接头突触间隙中乙酰胆碱积累，从而引起肌肉持续激活并最终导致麻痹。涕灭威诱导的麻痹试验是一种简单快速的方法，用于确定感兴趣的突变体的突触传递是否发生改变。该试验基于神经递质释放速率与麻痹速率之间的相关性。在本方案中，我们描述了一种同时评估不同基因型动物涕灭威敏感性的方法。
