Centre for DNA Nanotechnology (CDNA), at Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark, Phone: +45-87156729, Fax: +45-87154041; School of Physical Science and Technology, Lanzhou University, Lanzhou 730000, PR China.
Small. 2013 Sep 9;9(17):2954-9. doi: 10.1002/smll.201202861. Epub 2013 Feb 22.
The Watson-Crick base-pairing with specificity and predictability makes DNA molecules suitable for building versatile nanoscale structures and devices, and the DNA origami method enables researchers to incorporate more complexities into DNA-based devices. Thermally controlled atomic force microscopy in combination with nanomechanical spectroscopy with forces controlled in the pico Newton (pN) range as a novel technique is introduced to directly investigate the kinetics of multistrand DNA hybridization events on DNA origami nanopores under defined isothermal conditions. For the synthesis of DNA nanostructures under isothermal conditions at 60 °C, a higher hybridization rate, fewer defects, and a higher stability are achieved compared to room-temperature studies. By quantifying the assembly times for filling pores in origami structures at several constant temperatures, the fill factors show a consistent exponential increase over time. Furthermore, the local hybridization rate can be accelerated by adding a higher concentration of the staples. The new insight gained on the kinetics of staple-scaffold hybridization on the synthesis of two dimensional DNA origami structures may open up new routes and ideas for designing DNA assembly systems with increased potential for their application.
沃森-克里克碱基配对具有特异性和可预测性，使得 DNA 分子适合构建多功能的纳米结构和设备，而 DNA 折纸方法使研究人员能够将更多的复杂性纳入基于 DNA 的设备中。热控制原子力显微镜与纳米机械光谱学相结合，力在皮牛顿 (pN) 范围内可控，作为一种新的技术，被引入来直接研究在定义的等温条件下多链 DNA 在 DNA 折纸纳米孔上的杂交动力学。对于在 60°C 的等温条件下合成 DNA 纳米结构，与室温研究相比，实现了更高的杂交率、更少的缺陷和更高的稳定性。通过在几个恒温下定量填充折纸结构孔的组装时间，填充因子随时间呈一致的指数增长。此外，通过添加更高浓度的订书钉可以加速局部杂交率。在二维 DNA 折纸结构合成中，对订书钉-支架杂交动力学的新见解可能为设计具有更高应用潜力的 DNA 组装系统开辟新的途径和思路。
