Hassanpour Hossein, Bigham Sadegh Amin, Karimi Iraj, Heidari Khoei Heidar, Karimi Azarnoush, Edalati Shaarbaf Parinaz, Karimi Shayan Tahereh
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran.
Department of Biomedical Sciences, School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamilnadu-632014, India.
Int J Fertil Steril. 2017 Oct;11(3):148-155. doi: 10.22074/ijfs.2017.5020. Epub 2017 Aug 27.
This study was designed to create an experimental varicocele model by a simple surgical procedure in dog with minimum invasion and to investigate the effect of varicocele-induced infertility on the expression of six related genes (HSP90, HSP70, IL- 4, TNF, KITLG and KIT receptor).
In this experimental study, the proximal part of the pampiniform plexus of dog testes was partially occluded without abdominal incision which was confirmed by venographic examination. To evaluate varicocele in its acute form, dogs were castrated after 15 days and testes were dissected. Histopathologic evaluation was undertaken and the relative expression of the six genes was assessed by quantitative realtime polymerase chain reaction (PCR).
Microscopic changes showed tubule degeneration. The Johnson score was significantly decreased in the varicocele testes when compared with non-varicocele testes. Expressions of HSP90, TNF, KITLG and the KIT-receptor gene were significantly downregulated (P=0.029, 0.047, 0.004 and 0.035 respectively) in varicocele-induced testes while HSP70 was upregulated (P=0.018). IL-4 did not show differential expression (P=0.377).
We conclude that partial occlusion of the proximal part of the pampiniform plexus induces varicocele in the testis of dog. Differential expression of the mentioned genes may be responsible for the pathophysiology of varicocele and related subfertility.
本研究旨在通过一种简单的手术方法在犬类中创建一个微创的实验性精索静脉曲张模型，并研究精索静脉曲张所致不育对六个相关基因（热休克蛋白90、热休克蛋白70、白细胞介素-4、肿瘤坏死因子、干细胞因子配体和干细胞因子受体）表达的影响。
在本实验研究中，在不做腹部切口的情况下部分阻断犬睾丸蔓状静脉丛的近端，通过静脉造影检查予以证实。为评估急性形式的精索静脉曲张，15天后对犬实施去势并解剖睾丸。进行组织病理学评估，并通过定量实时聚合酶链反应（PCR）评估六个基因的相对表达。
显微镜下变化显示小管变性。与非精索静脉曲张睾丸相比，精索静脉曲张睾丸的约翰逊评分显著降低。在精索静脉曲张所致睾丸中，热休克蛋白90、肿瘤坏死因子、干细胞因子配体和干细胞因子受体基因的表达显著下调（分别为P = 0.029、0.047、0.004和0.035），而热休克蛋白70上调（P = 0.018）。白细胞介素-4未显示差异表达（P = 0.377）。
我们得出结论，蔓状静脉丛近端的部分阻断可诱发犬睾丸的精索静脉曲张。上述基因的差异表达可能是精索静脉曲张及相关亚生育状态病理生理学的原因。
