使用RNAscope和免疫组织化学评估肝细胞癌中血小板源性生长因子受体β的表达——初步报告
Evaluation of Podoplanin Expression in Hepatocellular Carcinoma Using RNAscope and Immunohistochemistry - A Preliminary Report.
作者信息
Cioca Andreea, Cimpean Anca Maria, Ceausu Raluca Amalia, Tarlui Valeria, Toma Alina, Marin Irina, Raica Marius
机构信息
Department of Histology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania
Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
出版信息
Cancer Genomics Proteomics. 2017 Sep-Oct;14(5):383-387. doi: 10.21873/cgp.20048.
BACKGROUND
Podoplanin (PDPN), a mucin-type transmembrane glycoprotein, is expressed in a variety of human cancer types, and contributes to tumor progression. Our goal was to evaluate PDPN expression in hepatocellular carcinoma (HCC) using both immunohistochemistry (IHC) and RNAscope in situ hybridization.
MATERIALS AND METHODS
Twenty patients with HCC who underwent partial hepatectomy with curative intent were retrospectively analyzed.
RESULTS
IHC gave positive results in 11 cases, while RNAscope assay for PDPN detected amplification in 16 cases. A significant association was noted between PDPN protein expression and histological tumor grade (p=0.036). Four cases that had negative PDPN results by RNAscope were also negative by IHC, while the remaining five cases with negative results by IHC were positive by RNAscope. A positive relationship was found between PDPN mRNA protein expression (p<0.001).
CONCLUSION
Our preliminary results suggest that PDPN contributes to the malignant potential of HCC. RNAscope proved to be a more sensitive and reliable method than IHC in PDPN detection.
背景
血小板反应蛋白-1结合蛋白（PDPN）是一种粘蛋白型跨膜糖蛋白，在多种人类癌症类型中表达，并促进肿瘤进展。我们的目标是使用免疫组织化学（IHC）和RNAscope原位杂交技术评估肝细胞癌（HCC）中PDPN的表达。
材料与方法
回顾性分析20例接受根治性部分肝切除术的HCC患者。
结果
免疫组织化学在11例中呈阳性结果，而PDPN的RNAscope检测在16例中检测到扩增。PDPN蛋白表达与组织学肿瘤分级之间存在显著相关性（p = 0.036）。RNAscope检测PDPN结果为阴性的4例免疫组织化学结果也为阴性，而免疫组织化学结果为阴性的其余5例RNAscope检测为阳性。PDPN mRNA蛋白表达之间存在正相关关系（p < 0.001）。
结论
我们的初步结果表明，PDPN有助于HCC的恶性潜能。在检测PDPN方面，RNAscope被证明是一种比免疫组织化学更敏感、更可靠的方法。
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