Tomescu Larisa Cristina, Cosma Andrei Alexandru, Fenesan Mihaela Pasca, Melnic Eugen, Petrovici Vergil, Sarb Simona, Chis Monica, Sas Ioan, Ribatti Domenico, Cimpean Anca Maria, Dorobantu Florica Ramona
Doctoral School in Medicine, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania.
Department of Obstetrics and Gynecology, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania.
Curr Issues Mol Biol. 2024 May 24;46(6):5161-5177. doi: 10.3390/cimb46060310.
The expression and function of podoplanin (PDPN) in the normal human placenta has been debated in placental evaluation. This study emphasizes the importance of a multimodal approach of PDPN expression in normal human placentas. A complete examination is performed using immunohistochemistry, RNAscope and automated Digital Image examination (DIA) interpretation. QuPath DIA-based analysis automatically generated the stromal and histological scores of PDPN expression for immunohistochemistry and RNAscope stains. The umbilical cord's isolated fibroblasts and luminal structures expressed PDPN protein and PDPN_mRNA. RNAscope detected PDPN_mRNA upregulation in syncytial placental knots trophoblastic cells, but immunohistochemistry did not certify this at the protein level. The study found a significant correlation between the IHC and RNAscope H-Score ( = 0.033) and Allred Score ( = 0.05). A successful multimodal strategy for PDPN assessment in human placentas confirmed PDPN expression heterogeneity in the full-term human normal placenta and umbilical cord at the protein and mRNA level. In placental syncytial knots trophoblastic cells, PDPN showed mRNA overexpression, suggesting a potential role in placenta maturation.
在胎盘评估中，人正常胎盘里血小板反应蛋白-1结构域蛋白（PDPN）的表达及功能一直存在争议。本研究强调了采用多模式方法研究PDPN在人正常胎盘中表达情况的重要性。运用免疫组织化学、RNAscope技术和自动化数字图像检查（DIA）解读进行全面检查。基于QuPath DIA的分析自动生成了免疫组织化学和RNAscope染色中PDPN表达的基质和组织学评分。脐带分离出的成纤维细胞和管腔结构表达PDPN蛋白和PDPN_mRNA。RNAscope检测到合体滋养层细胞形成的胎盘小结中PDPN_mRNA上调，但免疫组织化学在蛋白水平未证实这一点。该研究发现免疫组织化学和RNAscope的H评分（=0.033）以及奥尔雷德评分（=0.05）之间存在显著相关性。一种成功的多模式策略用于评估人胎盘中的PDPN，证实了足月人正常胎盘和脐带中PDPN在蛋白和mRNA水平上的表达异质性。在胎盘合体滋养层细胞形成的小结中，PDPN显示mRNA过表达，提示其在胎盘成熟过程中可能发挥作用。
