Liu Chuan, Wen Jian, Su Yong-Wen, Zhang Yu-Xin, Cai Xia, Zhang Yi
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Zhongguo Zhong Yao Za Zhi. 2016 Feb;41(4):586-591. doi: 10.4268/cjcmm20160406.
This study is to establish an HPLC fingerprint and quantitative analysis of 3 components of Gyantse Seabuckthorn from different producing areas.The separation was developed on Shimadzu InertSustain C18column (4.6 mm × 250 mm,5 μm) by gradient elution with acetonitrile and 0.2% phosphoric acid water as mobile phase at a flow rate of 1.0 mL•min ⁻¹; the detection wavelength was set at 360 nm and column temperature was set at 30 ℃. The data calculation was performed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(Version 2004A).The fingerprints of 10 batches of Gyantse Seabuckthorn were carried out by similarity comparison, and 12 chromatographic peaks were extracted as the common peaks of fingerprint, of which three main active ingredients were successfully determined. This is the first established fingerprint and multi-component quantitative determination of Gyantse Seabuckthorn by using HPLC. This method has good precision stability and repeatability that could provide basis for quality control and evaluation of Gyantse Seabuckthorn.
本研究旨在建立不同产地江孜沙棘的高效液相色谱指纹图谱及3种成分的定量分析方法。采用岛津InertSustain C18柱（4.6 mm×250 mm，5μm），以乙腈和0.2%磷酸水为流动相进行梯度洗脱，流速为1.0 mL•min⁻¹；检测波长设定为360 nm，柱温设定为30℃。采用《中药色谱指纹图谱相似度评价系统》（2004A版）进行数据计算。对10批江孜沙棘的指纹图谱进行相似度比较，提取12个色谱峰作为指纹图谱的共有峰，其中成功测定了3种主要活性成分。这是首次采用高效液相色谱法建立江孜沙棘的指纹图谱及多成分定量测定方法。该方法具有良好的精密度、稳定性和重复性，可为江孜沙棘的质量控制和评价提供依据。
