不同品种枳实的高效液相色谱指纹图谱及化学成分差异研究
[Study on HPLC fingerprint and chemical constituent difference of different species of Aurantii Fructus Immaturus].
作者信息
Zeng Hong-Lian, Liu Zhen-Li, Song Zhi-Qian, Wang Chun, Dong Yun-Zhuo, Ning Zhang-Chi, Zhao Si-Yu, Shu Yi-Song, He Dan, Wang Meng-Lei, Gan Jia-He, Liu Yuan-Yan
机构信息
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2016 Sep;41(17):3272-3278. doi: 10.4268/cjcmm20161725.
This study is to establish an HPLC fingerprint by HPLC-DAD method and simultaneous quantitative analysis of 17 components of 18 batches of Citrus aurantium and 10 batches of C. sinensis. The separation was performed on an Agilent Poroshell 120 SB-C₁₈ (4.6 mm×100 mm,2.7 μm) column with the gradient elution of methanol-0.1% formic acid water, the flow was 0.6 mL•min⁻¹. The detection wavelength was set at 318 nm. The column temperature was maintained at 30 ℃. The data calculation was performed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004A) together with SIMCA-P 13.0 software to clarify the differential marker between these two different species of Aurantii Fructus Immaturus. This method has good precision stability and repeatability that could provide basis for quality control and evaluation of Aurantii Fructus Immaturus.
本研究旨在采用高效液相色谱-二极管阵列检测法（HPLC-DAD）建立枳实和甜橙的高效液相色谱指纹图谱，并同时对18批枳实和10批甜橙中的17种成分进行定量分析。采用安捷伦Poroshell 120 SB-C₁₈（4.6 mm×100 mm，2.7 μm）色谱柱，以甲醇-0.1%甲酸水梯度洗脱，流速为0.6 mL•min⁻¹。检测波长设定为318 nm。柱温保持在30℃。采用中药色谱指纹图谱相似度评价系统（2004A版）和SIMCA-P 13.0软件进行数据计算，以明确这两种不同品种枳实之间的差异标志物。该方法具有良好的精密度、稳定性和重复性，可为枳实的质量控制和评价提供依据。
Zotero 插件