Department of Otorhinolaryngology, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, People's Republic of China.
Biosci Rep. 2020 Jun 26;40(6). doi: 10.1042/BSR20191370.
The study was aimed to investigate the protective effect of Asarum sieboldii Miq. essential oil (AEO) on ovalbumin (OVA)-induced allergic rhinitis (AR) in rats.
Sixty Sprague-Dawley male rats were randomly divided into six groups (n=10): control, model, cetirizine (Cet, 4.65 g/kg), and AEO (0.5, 1.5, 3 g/kg) groups. All animals except the control group received repeated intranasal instillation with 20 μl of 20% OVA in Al(OH)3 saline solvent for 15 days. The control group was intranasally instilled with 5 mg/ml of Al(OH)3 instead of the same procedure. In the 15 days, Cet and AEO were orally administrated for 28 days. At the end of the drug administration, 20 μl of 5% OVA was given to animals to stimulate allergic reaction, then the rat behavioral detection, assessment of the patho-morphological changes in nasal mucosa, and the serum biomarkers were determined. The result showed that AEO could significantly reduce the amount of nasal secretions, sneezing, and the degree of nasal scratching in AR rats with EC50 = 1.5 and 2.8 g/kg, respectively. The degree of nasal mucosal inflammation in AEO group improved, the levels of immunoglobulin E (IgE), histamine, IL-4, IL-5, IL-17 were decreased, and the level of IFN-γ was increased obviously with EC50 = 2 g/kg.
The study suggested that the possible mechanism might be related with the inhibition of histamine release and regulation of the cytokine levels, which plays an important role in the treatment of AR.
本研究旨在探讨细辛挥发油（AEO）对卵清蛋白（OVA）诱导的大鼠过敏性鼻炎（AR）的保护作用。
60 只雄性 Sprague-Dawley 大鼠随机分为 6 组（n=10）：对照组、模型组、西替利嗪（Cet，4.65 g/kg）组和 AEO（0.5、1.5、3 g/kg）组。除对照组外，所有动物均接受 20 μl 20%OVA 在 Al(OH)3 盐溶剂中的重复鼻内滴注，共 15 天。对照组以 5mg/ml 的 Al(OH)3 鼻内滴注代替相同程序。在 15 天内，Cet 和 AEO 连续口服给药 28 天。给药结束后，给动物滴注 20 μl 5%OVA 以刺激过敏反应，然后测定大鼠行为检测、鼻黏膜病理形态变化及血清生物标志物。结果表明，AEO 可显著减少 AR 大鼠的鼻分泌物量、打喷嚏和鼻搔抓次数，EC50 值分别为 1.5 和 2.8 g/kg。AEO 组鼻黏膜炎症程度改善，免疫球蛋白 E（IgE）、组胺、IL-4、IL-5、IL-17 水平降低，IFN-γ 水平明显升高，EC50 值为 2 g/kg。
研究表明，其可能的机制与抑制组胺释放和调节细胞因子水平有关，这在 AR 的治疗中起着重要作用。
