Chen Hongxia, Wang Chengzhang, Zhou Hao, Tao Ran, Ye Jianzhong, Li Wenjun
a Institute of Chemical Industry of Forest Products , CAF , Nanjing , China.
b Key and Open Laboratory on Forest Chemical Engineering , SFA , Nanjing , China.
Nat Prod Res. 2018 Sep;32(18):2221-2224. doi: 10.1080/14786419.2017.1366483. Epub 2017 Sep 5.
Three polysaccharides, LTPS-1, LTPS-21 and LTPS-31 were isolated and purified from the seed cakes of lacquer tree using DEAE-52 cellulose and Sephadex G-200 column chromatography. The total sugar contents of LTPS-1, LTPS-21 and LTPS-31 were 931.8, 958.2 and 895.1 g kg, respectively. LTPS-1 (3.48 kDa) was mainly composed of rhamnose, arabinose, glucose and galactose in a ratio of 35.36:5.06:1:2. LTPS-21 (11.4 kDa) was mainly composed of rhamnose, arabinose, mannose and galactose in a ratio of 41.93:21.8:1.01:9.24. LTPS-31 (19.49 kDa) was mainly composed of rhamnose, arabinose and mannose in a ratio of 38.31:16.44:1.1. IR analysis suggested they contained lower sulphuric acids, the LTPS-21 and LTPS-31 belonged to β-type polysaccharide. Among the three polysaccharides, LTPS-21 exhibited the strongest reducing power, scavenging activity on ABTS and hydroxyl radicals. These findings suggested that polysaccharides from the seed cakes could be potentially developed as natural functional ingredients in the food and cosmetic industry.
采用DEAE - 52纤维素和Sephadex G - 200柱色谱法从漆树籽饼中分离纯化出三种多糖，即LTPS - 1、LTPS - 21和LTPS - 31。LTPS - 1、LTPS - 21和LTPS - 31的总糖含量分别为931.8、958.2和895.1 g/kg。LTPS - 1（3.48 kDa）主要由鼠李糖、阿拉伯糖、葡萄糖和半乳糖组成，比例为35.36:5.06:1:2。LTPS - 21（11.4 kDa）主要由鼠李糖、阿拉伯糖、甘露糖和半乳糖组成，比例为41.93:21.8:1.01:9.24。LTPS - 31（19.49 kDa）主要由鼠李糖、阿拉伯糖和甘露糖组成，比例为38.31:16.44:1.1。红外光谱分析表明它们含较低的硫酸基团，LTPS - 21和LTPS - 31属于β型多糖。在这三种多糖中，LTPS - 21表现出最强的还原力、对ABTS和羟基自由基的清除活性。这些研究结果表明，籽饼中的多糖有可能被开发成为食品和化妆品工业中的天然功能成分。
