Järvi R, Pelto-Huikko M, Helen P, Hervonen A
Cell Tissue Res. 1987 Jul;249(1):1-5. doi: 10.1007/BF00215411.
The localization of somatostatin-like immunoreactivity (SOM-LI) was examined in human lumbar sympathetic ganglia using the peroxidase-antiperoxidase method. Few of the principal neurons showed immunolabelling for somatostatin and sparse networks of nerve terminals were unevenly associated with ganglion cells. Using light microscopy, the most intense SOM-LI was seen in the perinuclear zone of the neurons. Electron-microscopically, the staining was localized on the membranes of the Golgi apparatuses. In the nerve terminals, SOM-LI was seen inside the small vesicles (40-60 nm diameter). All neurons with SOM-LI were also found to be tyrosine-hydroxylase immunoreactive when examined with a double-staining technique. These results provide evidence that somatostatin and noradrenaline co-exist in human sympathetic neurons.
采用过氧化物酶-抗过氧化物酶法，对人腰交感神经节中生长抑素样免疫反应性（SOM-LI）的定位进行了研究。很少有主神经元显示出对生长抑素的免疫标记，且稀疏的神经终末网络与神经节细胞的关联并不均匀。在光学显微镜下，神经元的核周区域可见最强的SOM-LI。在电子显微镜下，染色定位于高尔基体的膜上。在神经终末，SOM-LI可见于小囊泡（直径40 - 60 nm）内。当采用双重染色技术检测时，所有具有SOM-LI的神经元也被发现对酪氨酸羟化酶呈免疫反应性。这些结果提供了证据，表明生长抑素和去甲肾上腺素共存于人类交感神经元中。
