体外组织氧化过程中14CO2释放量的测定；Kontes孔板的替代方法。

Measurement of 14CO2 evolution during tissue oxidation in vitro; an alternative to the Kontes well.

Shand J H, Noble R C

Comp Biochem Physiol A Comp Physiol. 1987;87(3):583-5. doi: 10.1016/0300-9629(87)90365-3.

Abstract

An alternative method to the use of the disposable Kontes well for trapping 14CO2 produced in the course of biological oxidations is described. 2. A polyethylene miniature scintillation vial was used to contain the hyamine hydroxide-impregnated filter paper wick. 3. The two methods are compared in their abilities to trap 14CO2 produced directly by acidification of sodium [14C]bicarbonate and during beta-oxidation of 1[14C] palmitic acid. 4. The miniature vial and Kontes well methods showed similar efficiencies in the trapping of 14CO2 (97% and 95%, respectively, on average) the radioactivity of which was determined in the miniature vial using 5 ml only of scintillation fluid compared with a minimum of 10 ml required by the standard scintillation vial used to accommodate the Kontes well. 5. The technical advantages of the suggested miniature vial system, during both incubation and counting stages, are discussed.

摘要

本文描述了一种替代使用一次性康宁管阱来捕获生物氧化过程中产生的14CO2的方法。2. 一个聚乙烯微型闪烁瓶用于容纳浸渍了氢氧化海胺的滤纸芯。3. 比较了这两种方法捕获由[14C]碳酸氢钠酸化直接产生的14CO2以及在1[14C]棕榈酸的β-氧化过程中产生的14CO2的能力。4. 微型瓶和康宁管阱方法在捕获14CO2方面显示出相似的效率（平均分别为97%和95%），其中14CO2的放射性在微型瓶中测定时仅使用5毫升闪烁液，而用于容纳康宁管阱的标准闪烁瓶至少需要10毫升。5. 讨论了所建议的微型瓶系统在孵育和计数阶段的技术优势。