Canabady-Rochelle Laetitia L S, Selmeczi Katalin, Collin Sabrina, Pasc Andreea, Muhr Laurence, Boschi-Muller Sandrine
Université de Lorraine, Laboratoire Réactions et Génie des Procédés (LRGP, UMR CNRS-UL 7274), France.
Université de Lorraine, Laboratoire Structure et Réactivité des Systèmes Moléculaires Complexes (SRSMC, UMR CNRS-UL 7565), Faculté des Sciences et Techniques, B.P. 70239, 54506 Vandoeuvre-lès-Nancy Cedex, France.
Food Chem. 2018 Jan 15;239:478-485. doi: 10.1016/j.foodchem.2017.06.116. Epub 2017 Jun 23.
There is a growing need in the industrial sector (health, nutrition and cosmetic) to discover new biomolecules with various physico-chemical and bioactive properties. Various beneficial effects of peptides - notably those produced from protein hydrolysis - are reported in the literature. The antioxidant activity involves various mechanisms, among them metal chelation, studied by UV-visible spectrophotometry. In this paper, we set up an original method of screening metal chelating peptides in a hydrolysate using Surface Plasmon Resonance (SPR) for their antioxidant properties. To date, the empirical approach used several cycles of hydrolysate fractionation and bioactivity evaluation until the isolation of the pure bioactive molecule and its identification. Besides, the detection of metal-chelating peptide is not sensitive enough by spectrophotometry. For the first time, metal chelating peptides were screened in hydrolysates using SPR and a correlation was established between affinity constant determined in SPR and metal chelation capacity determined from UV-visible spectrophotometry.
工业领域（健康、营养和化妆品行业）对发现具有各种物理化学和生物活性特性的新生物分子的需求日益增长。文献报道了肽的各种有益作用，尤其是那些由蛋白质水解产生的肽。抗氧化活性涉及多种机制，其中包括通过紫外可见分光光度法研究的金属螯合作用。在本文中，我们建立了一种使用表面等离子体共振（SPR）筛选水解产物中金属螯合肽抗氧化特性的原始方法。迄今为止，经验方法是对水解产物进行几个分级分离和生物活性评估循环，直到分离出纯生物活性分子并对其进行鉴定。此外，通过分光光度法检测金属螯合肽的灵敏度不够。首次使用SPR在水解产物中筛选金属螯合肽，并在SPR中测定的亲和常数与紫外可见分光光度法测定的金属螯合能力之间建立了相关性。
