Zapata José E, Gómez-Sampedro Leidy J
Nutrition and Food Technology Group, Faculty of Pharmaceutical and Food Sciences, University of Antioquia, Medellín 050010, Colombia.
Giepronal Group, School of Basic Sciences, Technology and Engineering, National Open and Distance University, Medellín 050023, Colombia.
Biotechnol Rep (Amst). 2024 Feb 28;42:e00832. doi: 10.1016/j.btre.2024.e00832. eCollection 2024 Jun.
The antioxidant and antiproliferative activity of red tilapia ( spp.) viscera hydrolysates (RTVH) was evaluated. For that, the hydrolysates was applied to three cancer cell lines (HepG2, Huh7 and SW480) and the control (CCD-18Co). Finally, the line on which the hydrolysate had the greatest effect (SW480) and the control (CCD-18Co) were subjected to the ApoTox-Glo Triplex Assay to determine apoptosis, toxicity, and cell viability. The result showed that hydrolysate had a dose-dependent cytotoxic effect selective on the three cancer cell lines, compared to the control cells. There is a relationship between the antioxidant capacity of RTVHs and their antiproliferative capacity on cancer cells evaluated, which achieved cell viability by action of RTVH of 34.68 and 41.58 and 25.41 %, to HepG2, Huh7 and SW480, respectively. The action of RTVH on cancer cell line SW480 is not due to the induction of apoptosis but to the rupture of the cell membrane.
评估了红罗非鱼（罗非鱼属）内脏水解物（RTVH）的抗氧化和抗增殖活性。为此，将水解物应用于三种癌细胞系（HepG2、Huh7和SW480）以及对照（CCD-18Co）。最后，对水解物作用最显著的细胞系（SW480）和对照（CCD-18Co）进行ApoTox-Glo三联检测，以确定细胞凋亡、毒性和细胞活力。结果表明，与对照细胞相比，水解物对三种癌细胞系具有剂量依赖性的细胞毒性作用。RTVH的抗氧化能力与其对所评估癌细胞的抗增殖能力之间存在关联，RTVH分别使HepG2、Huh7和SW480的细胞活力达到34.68%、41.58%和25.41%。RTVH对癌细胞系SW480的作用不是由于诱导细胞凋亡，而是由于细胞膜破裂。
