Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University Iksan Campus, Iksan 54596, South Korea.
Pathog Dis. 2017 Sep 29;75(7). doi: 10.1093/femspd/ftx094.
Brucella species are important etiological agents of zoonotic diseases. Attenuated Salmonella strains expressing Brucella abortus BCSP31, Omp3b and superoxide dismutase proteins were tested as vaccine candidates in this study. In order to determine the optimal dose for intraperitoneal (IP) inoculation required to obtain effective protection against brucellosis, mice were immunized with various doses of a mixture of the three vaccine strains. Fifty BALB/c mice were divided into five equal groups (groups A-E). Group A mice were intraperitoneally inoculated with 100 μL of sterile phosphate-buffered saline. Group B, C, D and E mice were intraperitoneally immunized with approximately 1.2 × 105 colony-forming units (CFU) mL-1 of Salmonella containing pMMP65 in 100 μL and with 1.2 × 104 CFU mL-1, 1.2 × 105 CFU mL-1 and 1.2 × 106 CFU mL-1 of the mixture of the three strains in 100 μL, respectively. Serum IgG, tumor necrosis factor alpha and interferon gamma concentrations were significantly higher in group E than in groups A-D. Following challenge with B. abortus 544, the challenge strain was not detected in the spleen of any mouse from group E. Thus, IP immunization with 1.2 × 106 CFU mL-1 of the mixture of the three vaccine strains induced immune responses and provided effective protection against brucellosis in mice.
布鲁氏菌属是重要的人畜共患病病原体。本研究以减毒鼠伤寒沙门氏菌表达布鲁氏菌 abortus BCSP31、Omp3b 和超氧化物歧化酶蛋白为候选疫苗进行了测试。为了确定腹腔内（IP）接种的最佳剂量，以获得有效的布鲁氏菌病保护，用三种疫苗株混合物的不同剂量免疫小鼠。将 50 只 BALB/c 小鼠分为五组（A-E 组）。A 组小鼠经腹腔内接种 100 μL 无菌磷酸盐缓冲液。B、C、D 和 E 组小鼠分别用 100 μL 含 pMMP65 的约 1.2×105 个菌落形成单位（CFU）mL-1的鼠伤寒沙门氏菌、1.2×104 CFU mL-1、1.2×105 CFU mL-1和 1.2×106 CFU mL-1的三种菌株混合物进行腹腔内免疫。E 组血清 IgG、肿瘤坏死因子-α和干扰素-γ浓度明显高于 A-D 组。用 B. abortus 544 攻毒后，E 组任何小鼠的脾脏均未检测到攻毒菌株。因此，用 1.2×106 CFU mL-1 的三种疫苗株混合物进行 IP 免疫可诱导免疫反应，并为小鼠提供有效的布鲁氏菌病保护。
