Liu Tiantian, Xie Feifei, Geng Lele, He Ruizhe, Sun Mengzhe, Ni Tao, Xu Peng, Xing Chao, Peng Yinbo, Chen Ke, Fang Yong
Department of Burns and Plastic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Int J Nanomedicine. 2025 Jan 18;20:771-789. doi: 10.2147/IJN.S506489. eCollection 2025.
The purpose of this study is to develop an innovative solution for chronic wounds in high-mobility areas, such as joints, where conventional treatments are hindered by passive healing mechanisms and the need for immobilization. By designing a micro-electro-Nanofiber dressing composed of piezoelectric polyvinylidene fluoride (PVDF) integrated with antimicrobial silver nanoparticles (AgNPs), this research aims to address the dual challenges of promoting effective wound healing and maintaining joint mobility.
Herein, we developed a novel micro-electro-Nanofiber dressing using electrospinning technology, incorporating polyvinylidene fluoride (PVDF) with silver nanoparticles (AgNPs). The optimized PVDF-AgNPs Nanofiber dressings exhibited strong piezoelectric effects suitable for joint wounds.
In vitro experiments demonstrated that the dressing effectively promoted fibroblast migration and collagen synthesis. In vivo, the dressing exhibited a trend of rapid healing in infected wounds within 12 days while modulating macrophage differentiation toward the anti-inflammatory M2 phenotype. Additionally, the incorporation of antimicrobial nanosilver effectively controlled local infections, further facilitating the healing process.
To sum up, by harnessing the piezoelectric effect to stimulate endogenous healing mechanisms without restricting joint mobility, the developed PVDF-AgNPs Nanofiber dressings represent a transformative approach for the treatment of wounds in highly mobile body areas.
本研究的目的是为关节等高活动部位的慢性伤口开发一种创新解决方案，在这些部位，传统治疗因被动愈合机制和固定需求而受到阻碍。通过设计一种由集成了抗菌银纳米颗粒（AgNPs）的压电聚偏二氟乙烯（PVDF）组成的微电纳米纤维敷料，本研究旨在应对促进有效伤口愈合和保持关节活动度这两个双重挑战。
在此，我们使用静电纺丝技术开发了一种新型微电纳米纤维敷料，将聚偏二氟乙烯（PVDF）与银纳米颗粒（AgNPs）结合。优化后的PVDF-AgNPs纳米纤维敷料表现出适用于关节伤口的强压电效应。
体外实验表明，该敷料能有效促进成纤维细胞迁移和胶原蛋白合成。在体内，该敷料在12天内对感染伤口呈现出快速愈合的趋势，同时调节巨噬细胞向抗炎M2表型分化。此外，抗菌纳米银的加入有效控制了局部感染，进一步促进了愈合过程。
综上所述，通过利用压电效应刺激内源性愈合机制而不限制关节活动度，所开发的PVDF-AgNPs纳米纤维敷料代表了一种用于治疗身体高活动部位伤口的变革性方法。
