Keshtkar Mohammad, Shahbazi-Gahrouei Daryoush, Khoshfetrat Seyyed Mehdi, Mehrgardi Masoud A, Aghaei Mahmoud
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Chemistry, University of Isfahan, Isfahan 81746, Iran.
J Med Signals Sens. 2016 Oct-Dec;6(4):243-247.
Early detection of breast cancer is the most effective way to improve the survival rate in women. Magnetic resonance imaging (MRI) offers high spatial resolution and good anatomic details, and its lower sensitivity can be improved by using targeted molecular imaging. In this study, AS1411 aptamer was conjugated to FeO@Au nanoparticles for specific targeting of mouse mammary carcinoma (4T1) cells that overexpress nucleolin. cytotoxicity of aptamer-conjugated nanoparticles was assessed on 4T1 and HFFF-PI6 (control) cells. The ability of the synthesized nanoprobe to target specifically the nucleolin overexpressed cells was assessed with the MRI technique. Results show that the synthesized nanoprobe produced strongly darkened T-weighted magnetic resonance (MR) images with 4T1 cells, whereas the MR images of HFFF-PI6 cells incubated with the nanoprobe are brighter, showing small changes compared to water. The results demonstrate that in a Fe concentration of 45 μg/mL, the nanoprobe reduced by 90% MR image intensity in 4T1 cells compared with the 27% reduction in HFFF-PI6 cells. Analysis of MR signal intensity showed statistically significant signal intensity difference between 4T1 and HFFF-PI6 cells treated with the nanoprobe. MRI experiments demonstrate the high potential of the synthesized nanoprobe as a specific MRI contrast agent for detection of nucleolin-expressing breast cancer cells.
早期发现乳腺癌是提高女性生存率的最有效方法。磁共振成像（MRI）具有高空间分辨率和良好的解剖细节，通过使用靶向分子成像可以提高其较低的灵敏度。在本研究中，AS1411适配体与FeO@Au纳米颗粒偶联，用于特异性靶向过表达核仁素的小鼠乳腺癌（4T1）细胞。评估了适配体偶联纳米颗粒对4T1和HFFF-PI6（对照）细胞的细胞毒性。用MRI技术评估了合成的纳米探针特异性靶向过表达核仁素细胞的能力。结果表明，合成的纳米探针在4T1细胞上产生了强烈变黑的T加权磁共振（MR）图像，而与纳米探针孵育的HFFF-PI6细胞的MR图像更亮，与水相比变化较小。结果表明，在铁浓度为45μg/mL时，纳米探针使4T1细胞的MR图像强度降低了90%，而HFFF-PI6细胞降低了27%。MR信号强度分析显示，用纳米探针处理的4T1和HFFF-PI6细胞之间的信号强度存在统计学显著差异。MRI实验证明了合成的纳米探针作为检测表达核仁素的乳腺癌细胞的特异性MRI造影剂具有很高的潜力。
