Laboratory of Histology Embryology and Reproductive Biology, Faculty of Medicine of Sfax, University of Sfax, Tunisia.
Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Tunisia.
Biomed Pharmacother. 2018 Jan;97:518-527. doi: 10.1016/j.biopha.2017.10.150. Epub 2017 Nov 6.
Imidacloprid (IMI) is a widely used in Tunisia and abroad, and high doses of IMI have been known to cause endocrine disruption. Some reports claim that Urtica urens L. (UU) can reduce toxicity thanks to it anti-inflammatory and antioxidant activities, but there is no scientific evidence justifying its use, which lets us think to its direct effect on the metabolism of the ovarian tissue. The present study was undertaken to evaluate the protective effect of UU against the toxicity of Confidor, whose active substance is imidacloprid (IMI), in female rat, as well as the chemical compositions of UU ethanol (EtOH) extract by GC-MS. Female rats were divided into control group, 3 groups treated with IMI at 50, 200 or 300mg/kg/day and three groups co-treated with IMI (50, 200 or 300mg/kg/day)+100mg/kg/day of UU, for 60days. Blood samples were collected for the dosage of 17β-estradiol levels. Ovaries were removed for tissular dosage of malondialdehyde (MDA), advanced oxidation protein products (AOPP), glutathione (GSH), vitamin E, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). Histological and histomorphometric examinations were performed as well. IMI caused an acute ovary injury, increased the ovary tissue levels of MDA and AOPP, and decreased the levels of GSH, vitamin E, and antioxidant enzyme activities. The number and the diameter of follicles were markedly diminished together with a reduction of the relative weight of ovaries. Compared with controls, the treated rats exhibited a significant reduction in serum 17b-estradiol levels. These results suggest an endocrine disruption by IMI which may interfere with ovarian follicles development in rat. The injection of UU EtOH extract improved the histological and all biochemical parameters cited above. In conclusion, IMI induced an acute ovary injury accompanied with disturbance of oxidant status and causes follicular atresia. Significant antioxidant activities were also observed in UU EtOH and a total of 31 compounds were identified. The injection of UU EtOH provided a significant protection which might be due to its antioxidant activities.
吡虫啉（IMI）在突尼斯国内外广泛使用，高剂量的 IMI 已被证明会导致内分泌干扰。一些报告声称，荨麻（UU）可以通过其抗炎和抗氧化活性来降低毒性，但没有科学证据证明其使用是合理的，这让我们想到它对卵巢组织代谢的直接影响。本研究旨在评估 UU 对杀虫剂吡虫啉（IMI）的毒性的保护作用，其活性物质为吡虫啉（IMI），并通过 GC-MS 分析 UU 乙醇（EtOH）提取物的化学成分。将雌性大鼠分为对照组、3 组分别用 IMI 处理 50、200 或 300mg/kg/天和 3 组用 IMI（50、200 或 300mg/kg/天）+100mg/kg/天 UU 处理，共 60 天。收集血液样本以测定 17β-雌二醇水平。取出卵巢进行丙二醛（MDA）、高级氧化蛋白产物（AOPP）、谷胱甘肽（GSH）、维生素 E、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的组织测定。还进行了组织学和组织形态学检查。IMI 导致卵巢急性损伤，增加卵巢组织 MDA 和 AOPP 水平，降低 GSH、维生素 E 和抗氧化酶活性。卵泡数量和直径明显减少，卵巢相对重量减轻。与对照组相比，处理组大鼠血清 17b-雌二醇水平显著降低。这些结果表明 IMI 可能通过干扰大鼠卵巢卵泡发育而导致内分泌干扰。UU EtOH 提取物的注射改善了上述所有组织学和生化参数。总之，IMI 诱导了卵巢急性损伤，伴随着氧化应激状态的紊乱，并导致卵泡闭锁。UU EtOH 还观察到显著的抗氧化活性，共鉴定出 31 种化合物。UU EtOH 的注射提供了显著的保护作用，这可能是由于其抗氧化活性。
