Humphrey Christine E, Burnett Nicole, Dubey Shivangi, Kyndt John A
College of Science and Technology, Bellevue University, Bellevue, NE 68005, USA.
Microorganisms. 2022 Oct 13;10(10):2024. doi: 10.3390/microorganisms10102024.
The genus is known for its versatile metabolic capabilities and has been proposed to be used in a wide variety of innovative applications, ranging from biohydrogen and electricity production, bioremediation and as biostimulant in agriculture. Here, we report the isolation, characterization and genome sequence analysis of a novel species, strain HC1, isolated from the Hell Creek urban native restoration area. Whole genome-based analysis, average nucleotide identity (ANI) comparison, and growth characteristics identified this isolate as a new species of the genus, for which we propose the name sp. nov. Besides containing several nitrogenases for nitrogen fixation and hydrogen production, the HC1 genome encodes a unique gene cluster, not found in any other species, which encodes genes for the degradation of yet-unidentified aromatic PCB-type chemicals with potentially interesting biotechnological applications. The genomic features of HC1 indicate that it plays a positive role in the degradation of anthropogenic substances and aids the restoration of the Hell Creek watershed by contributing to N and carbon fixation and plant growth; however, the genome also contains several antibiotic resistance genes, indicating a broad range of antibiotic resistance in this environmental isolate.
该属以其多样的代谢能力而闻名，并已被提议用于广泛的创新应用，包括生物制氢和发电、生物修复以及作为农业中的生物刺激剂。在此，我们报告了从地狱溪城市原生恢复区分离出的一种新型[属名]物种菌株HC1的分离、表征及基因组序列分析。基于全基因组的分析、平均核苷酸同一性（ANI）比较以及生长特性确定该分离株为[属名]属的一个新物种，我们为此提议命名为[新物种名]。除了含有几种用于固氮和产氢的固氮酶外，HC1基因组编码一个独特的基因簇，在任何其他[属名]物种中均未发现，该基因簇编码用于降解尚未鉴定的芳香族多氯联苯类化学物质的基因，具有潜在有趣的生物技术应用。[属名]HC1的基因组特征表明，它在人为物质的降解中发挥积极作用，并通过促进氮和碳的固定以及植物生长来帮助地狱溪流域的恢复；然而，该基因组还包含几个抗生素抗性基因，表明这种环境分离株具有广泛的抗生素抗性。
