Cui Heng-Lin, Lü Zhen-Zhen, Li Yang, Zhou Yao
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3622-3626. doi: 10.1099/ijsem.0.002182. Epub 2017 Sep 6.
A halophilic archaeal strain, YGH44T, was isolated from the Yinggehai marine solar saltern in Hainan Province of China. Cells were rod-shaped, stained Gram-negative and formed red-pigmented colonies on agar plates. Optimal growth was obtained with 3.4 M NaCl (range: 2.6-4.8 M), 0.5 M MgCl2 (range: 0.005-1.0 M), at 37 °C (range: 25-55 °C) and at pH 7.0 (range: pH 5.0-9.0). The cells lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was 1.7 M. Phylogenetic tree reconstructions based on 16S rRNA genes and rpoB' genes revealed that strain YGH44T was distinct from the related genera, Halovenus, Halapricum, Halorientalis, Halorhabdus and Halosimplex of the order Halobacteriales. The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and three unidentified glycolipids. The DNA G+C content of strain YGH44Twas 69.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YGH44T (=CGMCC 1.12234T=JCM 18646T) represents a novel species of a new genus within the order Halobacteriales, for which the name Salinirussus salinus gen. nov., sp. nov. is proposed.
从中国海南省莺歌海盐场分离出一株嗜盐古菌，命名为YGH44T。细胞呈杆状，革兰氏染色阴性，在琼脂平板上形成红色菌落。在3.4 M NaCl（范围：2.6 - 4.8 M）、0.5 M MgCl2（范围：0.005 - 1.0 M）、37 °C（范围：25 - 55 °C）和pH 7.0（范围：pH 5.0 - 9.0）条件下生长最佳。细胞在蒸馏水中会裂解，防止细胞裂解的最低NaCl浓度为1.7 M。基于16S rRNA基因和rpoB'基因构建的系统发育树显示，菌株YGH44T与盐杆菌目相关属Halovenus、Halapricum、Halorientalis、Halorhabdus和Halosimplex不同。该菌株的主要极性脂类为磷脂酰甘油、磷脂酰甘油磷酸甲酯和三种未鉴定的糖脂。菌株YGH44T的DNA G + C含量为69.0 mol%。表型、化学分类和系统发育特性表明，菌株YGH44T（=CGMCC 1.12234T=JCM 18646T）代表盐杆菌目内一个新属的一个新物种，为此提出新属名Salinirussus，新种名salinus，即盐栖盐古菌（Salinirussus salinus gen. nov., sp. nov.）。
