Kang Li, Yang Chunhong, Wu Haizhen, Chen Qiuyue, Huang Libo, Li Xianyao, Tang Hui, Jiang Yunliang
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University , Taian, People's Republic of China .
DNA Cell Biol. 2017 Nov;36(11):922-929. doi: 10.1089/dna.2017.3863. Epub 2017 Sep 6.
Ovarian theca cells play an indispensable role in ovarian follicular development and hormone secretion. miR-26a-5p was reported to be differentially expressed in mature and immature chicken ovaries in our previous study; however, the role of miR-26a-5p in regulating ovarian follicle function is still unclear. In this study, we demonstrated that the expression dynamics of TNRC6A mRNA in either chicken ovaries or follicles showed an opposite trend compared with that of chicken miR-26a-5p expression. miR-26a-5p inhibited TNRC6A mRNA expression by directly targeting its 3'-untranslated region in cultured chicken theca cells. Overexpression of miR-26a-5p promoted chicken follicular theca cell proliferation in vitro. Furthermore, overexpression of miR-26a-5p and knockdown of TNRC6A significantly upregulated the antiapoptotic BCL-2 gene. Taken together, this study revealed the expression dynamics of miR-26a-5p and TNRC6A in chicken ovaries and ovarian follicles and the relationship between the expression of miR-26a-5p and TNRC6A in chicken ovarian theca cells. These results suggest that miR-26a-5p facilitates chicken ovarian theca cell proliferation by targeting the TNRC6A gene.
卵巢膜细胞在卵泡发育和激素分泌过程中发挥着不可或缺的作用。在我们之前的研究中，据报道miR-26a-5p在成熟和未成熟鸡卵巢中的表达存在差异；然而，miR-26a-5p在调节卵泡功能中的作用仍不清楚。在本研究中，我们发现鸡卵巢或卵泡中TNRC6A mRNA的表达动态与鸡miR-26a-5p的表达呈现相反趋势。在培养的鸡膜细胞中，miR-26a-5p通过直接靶向TNRC6A mRNA的3'-非翻译区来抑制其表达。miR-26a-5p的过表达促进了体外鸡卵泡膜细胞的增殖。此外，miR-26a-5p的过表达和TNRC6A的敲低显著上调了抗凋亡BCL-2基因。综上所述，本研究揭示了miR-26a-5p和TNRC6A在鸡卵巢和卵泡中的表达动态，以及miR-26a-5p与TNRC6A在鸡卵巢膜细胞中的表达关系。这些结果表明，miR-26a-5p通过靶向TNRC6A基因促进鸡卵巢膜细胞增殖。
