Tanaka Mamoru, Suzuki Masanobu, Takei Yuichiro, Okamoto Takeaki, Watanabe Hiroyuki
Department of Nutrition, University of Kochi , Kochi, Kochi 781-8515, Japan.
Resources and Environment Division, Kochi Prefectural Industrial Technology Center , Kochi, Kochi 781-5101, Japan.
J Agric Food Chem. 2017 Sep 27;65(38):8386-8391. doi: 10.1021/acs.jafc.7b03322. Epub 2017 Sep 19.
We investigated whether Cirsium maritimum Makino can inhibit immunoglobulin-E-mediated allergic response in rat basophilic leukemia (RBL-2H3) cells and passive cutaneous anaphylaxis (PCA) in BALB/c mice. In vitro, the ethyl acetate extract of C. maritimum Makino (ECMM) significantly inhibited β-hexosaminidase release and decreased intracellular Ca levels in RBL-2H3 cells. Moreover, ECMM leaves more strongly suppressed the release of β-hexosaminidase than ECMM flowers. ECMM leaves also significantly suppressed the PCA reaction in the murine model. High-performance liquid chromatography and H and C nuclear magnetic resonance indicated that cirsimaritin, a flavonoid, was concentrated in active fractions of the extract. Our findings suggest that ECMM leaves have a potential regulatory effect on allergic reactions that may be mediated by mast cells. Furthermore, cirsimaritin may be the active anti-allergic component in C. maritimum Makino.
我们研究了滨海蓟（Cirsium maritimum Makino）是否能抑制大鼠嗜碱性白血病（RBL-2H3）细胞中免疫球蛋白E介导的过敏反应以及BALB/c小鼠的被动皮肤过敏反应（PCA）。在体外，滨海蓟的乙酸乙酯提取物（ECMM）显著抑制了RBL-2H3细胞中β-己糖胺酶的释放并降低了细胞内钙离子水平。此外，ECMM叶比ECMM花更强烈地抑制β-己糖胺酶的释放。ECMM叶在小鼠模型中也显著抑制了PCA反应。高效液相色谱以及氢和碳核磁共振表明，一种黄酮类化合物刺槐素集中在提取物的活性部分。我们的研究结果表明，ECMM叶对可能由肥大细胞介导的过敏反应具有潜在的调节作用。此外，刺槐素可能是滨海蓟中的活性抗过敏成分。
