Singh Rajnish Prakash, Runthala Ashish, Khan Shahid, Jha Prabhat Nath
Department of Biological Science, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
Indian Institute of Science, Bangalore, India.
PLoS One. 2017 Sep 6;12(9):e0183513. doi: 10.1371/journal.pone.0183513. eCollection 2017.
Salinity stress adversely affects the plant growth and is a major constraint to agriculture. In the present study, we studied the role of plant growth promoting rhizobacterium (PGPR) Enterobacter cloacae SBP-8 possessing ACC deaminase activity on proteome profile of wheat (Triticum aestivum L.) under high salinity (200 mM NaCl) stress. The aim of study was to investigate the differential expressed protein in selected three (T-1, T-2, T-3) treatments and absolute quantification (MS/MS analysis) was used to detect statistically significant expressed proteins. In this study, we investigated the adaptation mechanisms of wheat seedlings exposed to high concentration of NaCl treatment (200 mM) for 15 days in response to bacterial inoculation based on proteomic data. The identified proteins were distributed in different cellular, biological and molecular functions. Under salt stress, proteins related to ion-transport, metabolic pathway, protein synthesis and defense responsive were increased to a certain extent. A broader comparison of the proteome of wheat plant under different treatments revealed that changes in some of the metabolic pathway may be involved in stress adaption in response to PGPR inoculation. Hierarchical cluster analysis identified the various up-regulated/down-regulated proteins into tested three treatments. Our results suggest that bacterial inoculation enhanced the ability of wheat plant to combat salt stress via regulation of transcription factors, promoting antioxidative activity, induction of defense enzymes, lignin biosynthesis, and acceleration of protein synthesis.
盐胁迫对植物生长产生不利影响，是农业生产的主要限制因素。在本研究中，我们研究了具有ACC脱氨酶活性的植物促生根际细菌（PGPR）阴沟肠杆菌SBP-8对高盐（200 mM NaCl）胁迫下小麦（Triticum aestivum L.）蛋白质组图谱的作用。本研究的目的是调查选定的三种（T-1、T-2、T-3）处理中差异表达的蛋白质，并使用绝对定量（MS/MS分析）来检测具有统计学意义的表达蛋白。在本研究中，我们基于蛋白质组学数据，研究了接种细菌后，暴露于200 mM NaCl高浓度处理15天的小麦幼苗的适应机制。鉴定出的蛋白质分布在不同的细胞、生物学和分子功能中。在盐胁迫下，与离子转运、代谢途径、蛋白质合成和防御反应相关的蛋白质在一定程度上有所增加。对不同处理下小麦植株蛋白质组的更广泛比较表明，某些代谢途径的变化可能参与了对PGPR接种的应激适应。层次聚类分析将测试的三种处理中的各种上调/下调蛋白质进行了分类。我们的结果表明，接种细菌通过调节转录因子、促进抗氧化活性、诱导防御酶、木质素生物合成和加速蛋白质合成，增强了小麦植株对抗盐胁迫的能力。
