Zhang Rui, Bi Xin-Xing, Ye Ali, Kong Ling-Jun, Dou Ya-Ling
Department of Clinical Laboratory,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
Department of Clinical Laboratory,Xinhua Hospital of Beijing Tongzhou District,Beijing 101100,China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2017 Aug 20;39(4):506-510. doi: 10.3881/j.issn.1000-503X.2017.04.008.
Objective To investigate the prevalence of human papilloma virus(HPV)subtypes in patients and to provide an evidence for the prevention and treatment of HPV infection and the development of HPV vaccine. Methods Multiplex PCR was used to detect HPV DNA in 6917 patients in Peking Union Medical College Hospital from January 1,2013 to June 30,2015.Totally 5586 patients entered the final analysis after the repeat samples were deleted.The total positive rate of HPV subtypes(including high-risk subtypes including HPV-16,18,31,33,35,39,45,51,52,56,58,59,and 68 and low-risk subtypes including HPV-6 and 11)and the infection status of different age were analyzed. Results The total positive rate of HPV was 36.29%(2027/5586).The positive rate of high-risk subtype was 24.92%(1392/5586)and low-risk subtype was 1.66%(93/5586).The positive rate of multiple was 9.70%(542/5586)and multiple high-risk subtype was 7.75%(433/5586).The positive rate of high-risk subtype and multiple were 25.52%(1366/5353)and 11.16%(26/233)in female and 9.99%(535/5353)and 3.00%(7/233)in male,there were significantly difference(χ=24.61,χ=12.45,all P<0.001).The positive rate of low-risk subtypes(3.86%,9/233)in males was significantly higher than that in females(1.57%,84/5353)(χ=5.84,P=0.007).The high-risk HPV subtype infection mainly was seen in patients aged 31-50 years and the low-risk HPV subtype infection mainly in patients aged 21-40 years.The age of multiple HPV infections from 31-40 years.The lowest turn negative rates of subtype were HPV52 and HPV58.The top three HPV subtypes with the highest positive rates were HPV52,HPV16,and HPV58.Conclusions The positive rates of HPV type are different between male and female patients.The males are mainly infected with low-risk subtypes,whereas the females with high-risk subtypes and the multiple HPV subtypes.The top three high-risk subtypes are HPV-52,16,and 58.HPV subtypes with the lowest secondary negative rates are HPV-52 and 58.HPV infection is mainly seen in young individuals.
目的 调查患者中人乳头瘤病毒（HPV）亚型的流行情况，为HPV感染的防治及HPV疫苗的研发提供依据。方法 采用多重聚合酶链反应（PCR）检测2013年1月1日至2015年6月30日北京协和医院6917例患者的HPV DNA。剔除重复样本后，共5586例患者进入最终分析。分析HPV亚型（包括高危亚型HPV-16、18、31、33、35、39、45、51、52、56、58、59和68，以及低危亚型HPV-6和11）的总阳性率及不同年龄段的感染状况。结果 HPV总阳性率为36.29%（2027/5586）。高危亚型阳性率为24.92%（1392/5586），低危亚型阳性率为1.66%（93/5586）。多重感染阳性率为9.70%（542/5586），多重高危亚型阳性率为7.75%（433/5586）。高危亚型和多重感染在女性中的阳性率分别为25.52%（1366/5353）和11.16%（26/233），在男性中的阳性率分别为9.99%（535/5353）和3.00%（7/233），差异有统计学意义（χ=24.61，χ=12.45，均P<0.001）。男性低危亚型阳性率（3.86%，9/233）显著高于女性（1.57%，84/5353）（χ=5.84，P=0.007）。高危HPV亚型感染主要见于31-50岁患者，低危HPV亚型感染主要见于21-40岁患者。多重HPV感染年龄为31-40岁。亚型转阴率最低的是HPV52和HPV58。阳性率最高的前三位HPV亚型是HPV52、HPV16和HPV58。结论 男女患者HPV型别阳性率不同。男性主要感染低危亚型，而女性主要感染高危亚型和多重HPV亚型。高危前三位亚型是HPV-52、16和58。转阴率最低的HPV亚型是HPV-52和58。HPV感染主要见于年轻人。
