Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via Balzaretti 9, 20133, Milano, Italy.
Res Microbiol. 2019 Nov-Dec;170(8):366-373. doi: 10.1016/j.resmic.2019.07.005. Epub 2019 Aug 1.
The surface of the outer membrane of Gram-negative bacteria is covered by a tightly packed layer of lipopolysaccharide molecules which provide a barrier against many toxic compounds and antibiotics. Lipopolysaccharide, synthesized in the cytoplasm, is assembled in the periplasmic leaflet of the inner membrane where the intermembrane Lpt system mediates its transport to the cell surface. The first step of lipopolysaccharide transport is its extraction from the outer leaflet of inner membrane powered by the atypical LptBFGC ABC transporter. Here we review latest advances leading to understanding at molecular level how lipopolysaccharide is transported irreversibly to the outer membrane.
革兰氏阴性细菌外膜的表面覆盖着一层紧密排列的脂多糖分子，这层分子提供了一道屏障，防止许多有毒化合物和抗生素进入细胞。脂多糖在细胞质中合成，在内膜的周质层中组装，而跨膜 Lpt 系统则介导其向细胞表面的运输。脂多糖运输的第一步是通过非典型的 LptBFGC ABC 转运体从内膜的外叶中将其提取出来，该转运体提供动力。在这里，我们回顾了最新的进展，这些进展使我们能够在分子水平上理解脂多糖如何不可逆地运输到外膜。
