Nutrilite Health Institute, 720 Cailun Road, Shanghai 201203, China.
Nutrilite Health Institute, 7575 East Fulton Avenue, Ada, MI 49355, USA.
Nutrients. 2017 Sep 6;9(9):978. doi: 10.3390/nu9090978.
Gastritis or peptic ulcer is believed to affect about half of people worldwide. Traditional medications can lead to adverse effects, therefore, alternative nutritional strategies are needed to prevent the development of gastric mucosal damage. A novel combination of two food-grade ingredients, wheat peptides and fucoidan (WPF), was prepared to treat male Sprague Dawley rats for 30 days before gastric mucosal damage was induced by oral administration of ethanol. The serum levels of biomarkers were determined by enzyme-linked immunosorbent assay. Biomarkers in stomach tissue were analyzed using immunohistochemistry. In addition, human gastric epithelial cell line (GES-1) was used to investigate protein expression by Western blot. WPF could attenuate ethanol-induced gastric mucosal damage in an inverse dose-dependent manner, with both ulcer index and pathological index improved. WPF increased superoxide dismutase level and decreased malondialdehyde level. WPF also decreased the levels of interleukin-8, platelet-activating factor, and Caspase 3, while increasing the levels of prostaglandin E-2, epidermal growth factor (EGF), and EGF receptor (EGFR). Furthermore, phosphorylation of EGFR and extracellular signal-regulated kinases was induced by WPF in GES-1 cells. In conclusion, the novel combination of wheat peptides and fucoidan attenuated ethanol-induced gastric mucosal damage in rats through anti-oxidant, anti-inflammatory, and pro-survival mechanisms.
胃炎或消化性溃疡被认为影响着全球约一半的人。传统药物可能会导致不良反应，因此需要替代营养策略来预防胃黏膜损伤的发生。一种新型的两种食品级成分——小麦肽和褐藻糖胶（WPF）的组合，被用于治疗雄性 Sprague Dawley 大鼠，在口服乙醇诱导胃黏膜损伤之前，连续治疗 30 天。通过酶联免疫吸附试验测定血清生物标志物水平。采用免疫组织化学法分析胃组织中的生物标志物。此外，还用人胃上皮细胞系（GES-1）通过 Western blot 法研究蛋白表达。WPF 能够以剂量依赖的方式减轻乙醇诱导的胃黏膜损伤，溃疡指数和病理指数均得到改善。WPF 增加了超氧化物歧化酶的水平，降低了丙二醛的水平。WPF 还降低了白细胞介素-8、血小板激活因子和 Caspase 3 的水平，同时增加了前列腺素 E-2、表皮生长因子（EGF）和 EGF 受体（EGFR）的水平。此外，WPF 还诱导了 GES-1 细胞中 EGFR 和细胞外信号调节激酶的磷酸化。总之，小麦肽和褐藻糖胶的新型组合通过抗氧化、抗炎和促进生存的机制减轻了乙醇诱导的大鼠胃黏膜损伤。
