Heilongjiang Provincial Key Laboratory of Corn Deep Processing Theory and Technology, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China.
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Nutrients. 2023 Aug 5;15(15):3467. doi: 10.3390/nu15153467.
More than 50% of the world population is infected with (), which is classified as group I carcinogen by the WHO. surface adhesins specifically recognize gastric mucosal epithelial cells' (GES-1 cells) receptor to complete the adhesion. Blocking the adhesion with an anti-adhesion compound is an effective way to prevent infection. The present study found that corn protein hydrolysate, hydrolyzed by Neutral, effectively alleviated gastric injury induced by infection through anti-adhesive and anti-inflammatory effects in vitro and in vivo. The hydrolysate inhibited adhesion to GES-1 cells significantly, and its anti-adhesive activity was 50.44 ± 0.27% at 4 mg/mL, which indicated that the hydrolysate possessed a similar structure to the GES-1 cells' receptor, and exhibited anti-adhesive activity in binding to . In vivo, compared with the infection model group, the medium and high dose of the hydrolysate (400-600 mg/kg·bw) significantly decreased ( 0.05) the amount of colonization, pro-inflammatory cytokines (IL-6, IL-1β, TNF-α and MPO), chemokines (KC and MCP-1) as well as key metabolites of NF-κB signaling pathway levels (TLR4, MyD88 and NF-κB), and it increased antioxidant enzyme contents (SOD and GSH-Px) and the mitigation of -induced pathological changes in the gastric mucosa. Taken together, these results indicated that the hydrolysate intervention can prevent -induced gastric injury by anti-adhesive activity and inhibiting the NF-κB signaling pathway's induction of inflammation. Hence, the corn protein hydrolysate might act as a potential anti-adhesive agent to prevent infection.
世界上超过 50%的人口感染了幽门螺杆菌，它被世界卫生组织列为 I 类致癌物质。表面黏附素特异性识别胃黏膜上皮细胞（GES-1 细胞）的受体，完成黏附。用抗黏附化合物阻断黏附是预防幽门螺杆菌感染的有效方法。本研究发现，玉米蛋白经中性蛋白酶水解得到的水解产物在体外和体内通过抗黏附和抗炎作用有效缓解了幽门螺杆菌感染引起的胃损伤。该水解产物能显著抑制幽门螺杆菌对 GES-1 细胞的黏附，其在 4mg/mL 时的抗黏附活性为 50.44±0.27%，这表明该水解产物具有与 GES-1 细胞受体相似的结构，通过与幽门螺杆菌结合表现出抗黏附活性。在体内，与幽门螺杆菌感染模型组相比，水解产物的中、高剂量（400-600mg/kg·bw）显著降低（P<0.05）了定植量、促炎细胞因子（IL-6、IL-1β、TNF-α 和 MPO）、趋化因子（KC 和 MCP-1）以及 NF-κB 信号通路的关键代谢物水平（TLR4、MyD88 和 NF-κB），增加了抗氧化酶含量（SOD 和 GSH-Px），减轻了幽门螺杆菌引起的胃黏膜病理变化。综上所述，这些结果表明，水解产物干预可以通过抗黏附活性和抑制 NF-κB 信号通路诱导的炎症来预防幽门螺杆菌感染引起的胃损伤。因此，玉米蛋白水解产物可能作为一种潜在的抗黏附剂，用于预防幽门螺杆菌感染。
