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蜕皮触发激素信号传导（ETH/ETHR-A）是双翅目实蝇科幼虫-幼虫蜕皮所必需的。

Ecdysis Triggering Hormone Signaling (ETH/ETHR-A) Is Required for the Larva-Larva Ecdysis in (Diptera: Tephritidae).

作者信息

Shi Yan, Jiang Hong-Bo, Gui Shun-Hua, Liu Xiao-Qiang, Pei Yu-Xia, Xu Li, Smagghe Guy, Wang Jin-Jun

机构信息

Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest UniversityChongqing, China.

Academy of Agricultural Sciences, Southwest UniversityChongqing, China.

出版信息

Front Physiol. 2017 Aug 22;8:587. doi: 10.3389/fphys.2017.00587. eCollection 2017.

DOI:10.3389/fphys.2017.00587
PMID:28878684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5572281/
Abstract

Insects must undergo ecdysis for successful development and growth, and the ecdysis triggering hormone (ETH), released by the Inka cells, is a master hormone in this process. In this study, we determined the sequence of the ETH precursor and receptors in an agriculturally important pest insect, the oriental fruit fly (Hendel). We identified two functionally distinct splice receptor isoforms: BdETH-R-A and BdETH-R-B, and when expressed in Chinese hamster ovary (CHO-WTA11) cells, they exhibited a high sensitivity to the two mature peptides BdETH1 and BdETH2. The transcript was detected in the tracheal tissue of the larvae. Inka cells were identified with immunohistochemical antibody staining against ETH1, and hybridization with specific DNA probes. Selective RNA silencing of or , but not of , caused developmental failure at ecdysis. The dsRNA-treated larvae displayed tracheal defects and could not shed the old cuticle followed by death. Our results demonstrated that BdETH, via activation of BdETH-R-A but not ETH-R-B, plays an essential role in regulating the process of larva-larva ecdysis in .

摘要

昆虫必须经历蜕皮才能成功发育和生长，由印卡细胞释放的蜕皮触发激素（ETH）是这一过程中的主要激素。在本研究中，我们确定了农业重要害虫东方果实蝇（亨德尔）中ETH前体和受体的序列。我们鉴定出两种功能不同的剪接受体亚型：BdETH-R-A和BdETH-R-B，当它们在中国仓鼠卵巢（CHO-WTA11）细胞中表达时，对两种成熟肽BdETH1和BdETH2表现出高敏感性。该转录本在幼虫的气管组织中被检测到。通过针对ETH1的免疫组织化学抗体染色以及与特异性DNA探针杂交来鉴定印卡细胞。对BdETH-R-A或BdETH-R-B进行选择性RNA沉默，但不对BdETH进行沉默，会导致蜕皮时发育失败。经dsRNA处理的幼虫表现出气管缺陷，无法蜕去旧表皮，随后死亡。我们的结果表明，BdETH通过激活BdETH-R-A而非ETH-R-B，在调节东方果实蝇幼虫-幼虫蜕皮过程中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/4bb6dbe48ffd/fphys-08-00587-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/1387eb3d0511/fphys-08-00587-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/d2de24b2c10c/fphys-08-00587-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/e1e357173420/fphys-08-00587-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/a28192d810cb/fphys-08-00587-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/e0ba427d2c36/fphys-08-00587-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/225a8469135d/fphys-08-00587-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/a666814aa1ce/fphys-08-00587-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/4bb6dbe48ffd/fphys-08-00587-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/1387eb3d0511/fphys-08-00587-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/d2de24b2c10c/fphys-08-00587-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/e1e357173420/fphys-08-00587-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/a28192d810cb/fphys-08-00587-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/e0ba427d2c36/fphys-08-00587-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/225a8469135d/fphys-08-00587-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/a666814aa1ce/fphys-08-00587-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/5572281/4bb6dbe48ffd/fphys-08-00587-g0008.jpg

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