Cellslide自动液基细胞学系统在HIV阳性女性中的性能表现。

Performance of the Cellslide automated liquid-based cytology system amongst HIV-positive women.

作者信息

Michelow Pamela, Sherrin Amanda, Rossouw Louise, Mohaleamolla Samson, Evans Denise, Swarts Avril, Rakhombe Ntombiyenkosi, Smith Jennifer S, Firnhaber Cynthia

机构信息

Department of Anatomical Pathology, University of the Witwatersrand and National Health Laboratory Service, Johannesburg, South Africa.

Department of Internal Medicine, University of the Witwatersrand, Johannesburg, South Africa.

出版信息

Afr J Lab Med. 2016 Feb 1;5(1):278. doi: 10.4102/ajlm.v5i1.278. eCollection 2016.

DOI:10.4102/ajlm.v5i1.278

PMID:28879102

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5436391/

Abstract

BACKGROUND

Many women undergoing cervical screening as part of a national South African screening programme may be positive for HIV. The performance of liquid-based cytology (LBC) on samples from HIV-positive women needs to be determined.

OBJECTIVES

The performance of the Cellslide automated LBC system was evaluated as a possible alternative to conventional cytology in a national cervical cancer screening programme.

METHODS

Split samples from 348 HIV-positive women attending an HIV treatment clinic in Johannesburg, South Africa were examined by conventional cytology and monolayer LBC methods. All samples were stained, examined and reported in the same manner. Cytotechnologists were blinded to the conventional smear diagnosis if the LBC smear was screened and vice versa.

RESULTS

The same percentage of inadequate smears (1.4%) was obtained by conventional cytology and LBC. Atypical squamous cells of undetermined significance were observed in 5.2% of conventional smears and 4.0% of LBC smears. Low-grade squamous intraepithelial lesions were found in 35.6% of conventional smears and 32.7% of LBC smears. Only one conventional smear was categorised as atypical squamous cells - cannot exclude a high-grade lesion, whereas five such cases were identified on LBC. High-grade squamous intraepithelial lesions were seen in 21.6% of conventional smears and 23.3% LBC smears. No invasive carcinoma was identified.

CONCLUSION

The performance of the Cellslide LBC system was similar to that of conventional cytology in this population of high-risk HIV-positive women, indicating that it may be introduced successfully as part of a cervical cancer screening programme.

摘要

背景

在南非国家筛查项目中，许多接受宫颈筛查的女性可能为艾滋病毒阳性。需要确定液基细胞学（LBC）在艾滋病毒阳性女性样本上的表现。

目的

在国家宫颈癌筛查项目中，评估Cellslide自动LBC系统作为传统细胞学的一种可能替代方法的表现。

方法

对来自南非约翰内斯堡一家艾滋病毒治疗诊所的348名艾滋病毒阳性女性的样本进行分割，分别采用传统细胞学和单层LBC方法进行检查。所有样本均以相同方式染色、检查和报告。如果对LBC涂片进行筛查，则细胞技术人员对传统涂片诊断结果不知情，反之亦然。

结果

传统细胞学和LBC获得的涂片不合格率相同（1.4%）。在5.2%的传统涂片中观察到意义不明确的非典型鳞状细胞，在4.0%的LBC涂片中观察到此类细胞。在35.6%的传统涂片中发现低级别鳞状上皮内病变，在32.7%的LBC涂片中发现此类病变。只有一张传统涂片被归类为非典型鳞状细胞——不能排除高级别病变，而在LBC上发现了5例此类病例。在21.6%的传统涂片中观察到高级别鳞状上皮内病变，在23.3%的LBC涂片中观察到此类病变。未发现浸润性癌。