St Laurent Georges, Seilheimer Bernd, Tackett Michael, Zhou Jianhua, Shtokalo Dmitry, Vyatkin Yuri, Ri Maxim, Toma Ian, Jones Dan, McCaffrey Timothy A
St. Laurent InstituteVancouver, WA, United States.
SeqLL, Inc.Woburn, MA, United States.
Front Mol Biosci. 2017 Aug 17;4:57. doi: 10.3389/fmolb.2017.00057. eCollection 2017.
Wound healing involves an orchestrated response that engages multiple processes, such as hemostasis, cellular migration, extracellular matrix synthesis, and in particular, inflammation. Using a murine model of cutaneous wound repair, the transcriptome was mapped from 12 h to 8 days post-injury, and in response to a multicomponent, multi-target natural product, Tr14. Using single-molecule RNA sequencing (RNA-seq), there were clear temporal changes in known transcripts related to wound healing pathways, and additional novel transcripts of both coding and non-coding genes. Tr14 treatment modulated >100 transcripts related to key wound repair pathways, such as response to wounding, wound contraction, and cytokine response. The results provide the most precise and comprehensive characterization to date of the transcriptome's response to skin damage, repair, and multicomponent natural product therapy. By understanding the wound repair process, and the effects of natural products, it should be possible to intervene more effectively in diseases involving aberrant repair.
伤口愈合涉及一个精心编排的反应过程，该过程涉及多个步骤，如止血、细胞迁移、细胞外基质合成，尤其是炎症反应。利用皮肤伤口修复的小鼠模型，绘制了损伤后12小时至8天的转录组图谱，并研究了其对一种多成分、多靶点天然产物Tr14的反应。使用单分子RNA测序（RNA-seq），与伤口愈合途径相关的已知转录本存在明显的时间变化，同时还发现了编码基因和非编码基因的新转录本。Tr14处理调节了超过100个与关键伤口修复途径相关的转录本，如对伤口的反应、伤口收缩和细胞因子反应。这些结果提供了迄今为止对转录组对皮肤损伤、修复和多成分天然产物治疗反应的最精确和全面的表征。通过了解伤口修复过程以及天然产物的作用，应该有可能更有效地干预涉及异常修复的疾病。
