Freitas Rubens M, Spin-Neto Rubens, Spolidório Luiz Carlos, Campana-Filho Sergio Paulo, Marcantonio Rosemary Adriana C, Marcantonio Elcio
Department of Periodontology, Araraquara Dental School, Universidade Estadual Paulista, Rua Humaitá, 1680, CEP 14801-903, Araraquara, São Paulo, Brazil.
Department of Pathology, Araraquara Dental School, Universidade Estadual Paulista, 14801-903 Araraquara, São Paulo, Brazil.
Materials (Basel). 2011 Feb 10;4(2):380-389. doi: 10.3390/ma4020380.
Natural polymers, such as chitosan, obtained from chitin, are been widely studied for use in the tissue regeneration field. This study established a protocol to attain membranes made from this biopolymer, consisting of high or low molecular weight chitosan. The biocompatibility of these membranes was histologically evaluated, comparing them to collagen membrane surgically implanted in rat subcutaneous tissue. Fifteen Holtzmann rats were divided in three experimental groups: High and Low Molecular Weight Chitosan membranes (HMWC and LMWC) and Collagen membranes (C-control group); each of them with three experimental periods: 7, 15 and 30 days. As a result, after the seven days evaluation, the membranes were present and associated with a variable degree of inflammation, and after the 15 and 30 days evaluations, the membranes were absent in all groups. It is concluded that the chitosan-based membranes were successfully attained and presented comparable resorption times to collagen membranes.
从几丁质中获得的天然聚合物，如壳聚糖，已在组织再生领域得到广泛研究。本研究建立了一种制备由这种生物聚合物制成的膜的方案，该生物聚合物由高分子量或低分子量壳聚糖组成。通过组织学评估这些膜的生物相容性，并将它们与手术植入大鼠皮下组织的胶原膜进行比较。将15只霍尔兹曼大鼠分为三个实验组：高分子量和低分子量壳聚糖膜（HMWC和LMWC）以及胶原膜（C-对照组）；每组有三个实验周期：7天、15天和30天。结果，在7天评估后，膜存在且伴有不同程度的炎症，而在15天和30天评估后，所有组的膜均消失。得出的结论是，成功制备了基于壳聚糖的膜，其吸收时间与胶原膜相当。
