Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Cell Biol Int. 2018 Aug;42(8):923-930. doi: 10.1002/cbin.10870. Epub 2017 Oct 16.
Studies have shown that miR-4317 is dysregulated in tumor, but the biologic role of miR-4317 in tumor development and progression remains unknown. The present study aimed to investigate the role of miR-4317 in human gastric cancer. Quantitative real-time PCR was used to quantify miR-4317 expression levels in clinical gastric cancer specimens and cell lines. MTT, colony formation and cell cycle assays were performed to identify the contributions of miR-4317 to cell proliferation in gastric cancer cell lines. The results showed that miR-4317 was significantly decreased in 17 clinical gastric cancer specimens compared with adjacent non-tumor stomach tissues. Forced expression of miR-4317 suppressed gastric cancer cell proliferation and blocked S-G2/M transition. Bioinformatics and dual-luciferase reporter assays confirmed that ZNF322 is a direct target of miR-4317. Silencing ZNF322 recapitulated the cellular and molecular effects seen upon miR-4317 overexpression. These findings indicate that miR-4317 represses the proliferation of gastric cancer cell, at least in part, by targeting and suppressing ZNF322 and that it may serve as a therapeutic target for gastric cancer treatment.
研究表明 miR-4317 在肿瘤中失调，但 miR-4317 在肿瘤发生和发展中的生物学作用尚不清楚。本研究旨在探讨 miR-4317 在人胃癌中的作用。定量实时 PCR 用于定量检测临床胃癌标本和细胞系中 miR-4317 的表达水平。MTT、集落形成和细胞周期分析用于鉴定 miR-4317 对胃癌细胞系中细胞增殖的贡献。结果显示，与相邻非肿瘤胃组织相比，17 例临床胃癌标本中 miR-4317 显著降低。强制表达 miR-4317 抑制胃癌细胞增殖并阻断 S-G2/M 期转换。生物信息学和双荧光素酶报告基因实验证实 ZNF322 是 miR-4317 的直接靶标。沉默 ZNF322 可重现 miR-4317 过表达时观察到的细胞和分子效应。这些发现表明，miR-4317 通过靶向和抑制 ZNF322 抑制胃癌细胞的增殖，至少部分如此，并且它可能作为胃癌治疗的治疗靶点。
