Department of Biotechnology, Chonnam National University, Chonnam, 59626, Republic of Korea.
School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510275, China.
Sci Rep. 2017 Sep 7;7(1):10862. doi: 10.1038/s41598-017-11495-4.
Soybean is a promising biomass resource for generation of second-generation biofuels. Despite the utility of soybean cellulosic biomass and post-processing residues in biofuel generation, there is no comprehensive information available on cell wall loosening and degradation related gene families. In order to achieve enhanced lignocellulosic biomass with softened cell walls and reduced recalcitrance, it is important to identify genes involved in cell wall polymer loosening and degrading. Comprehensive genome-wide analysis of gene families involved in cell wall modifications is an efficient stratagem to find new candidate genes for soybean breeding for expanding biofuel industry. We report the identification of 505 genes distributed among 12 gene families related to cell wall loosening and degradation. 1262 tandem duplication events contributed towards expansion and diversification of studied gene families. We identified 687 Simple Sequence Repeat markers and 5 miRNA families distributed on 316 and 10 genes, respectively. Publically available microarray datasets were used to explore expression potential of identified genes in soybean plant developmental stages, 68 anatomical parts, abiotic and biotic stresses. Co-expression networks revealed transcriptional coordination of different gene families involved in cell wall loosening and degradation process.
大豆是生产第二代生物燃料的有前途的生物质资源。尽管大豆纤维质生物质和后处理残余物在生物燃料生产中具有实用性，但目前还没有关于细胞壁疏松和降解相关基因家族的综合信息。为了实现具有软化细胞壁和降低抗降解性的增强木质纤维素生物质，识别参与细胞壁聚合物疏松和降解的基因非常重要。全面的全基因组分析涉及细胞壁修饰的基因家族是寻找用于扩大生物燃料工业的大豆育种新候选基因的有效策略。我们报告了 505 个基因的鉴定，这些基因分布在与细胞壁疏松和降解相关的 12 个基因家族中。1262 个串联重复事件有助于研究基因家族的扩展和多样化。我们鉴定了 687 个简单序列重复标记和 5 个 miRNA 家族，分别分布在 316 个和 10 个基因上。利用公开的微阵列数据集，探讨了鉴定基因在大豆植物发育阶段、68 个解剖部位、非生物和生物胁迫下的表达潜力。共表达网络揭示了参与细胞壁疏松和降解过程的不同基因家族之间的转录协调。
