College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China.
Crop Science Department, Njala University, Njala Campus, Private Mail bag, Freetown 999127, Sierra Leone.
Int J Mol Sci. 2023 Jan 1;24(1):775. doi: 10.3390/ijms24010775.
The xyloglucan endotransglucosylase/hydrolase (XET/XEH, also named XTH) family is a multigene family, the function of which plays a significant role in cell-wall rebuilding and stress tolerance in plants. However, the specific traits of the gene family members and their expression pattern in different tissues and under stress have not been carried out in sweet potato. Thirty-six XTH genes were identified in all of which had conserved structures (Glyco_hydro_16). Based on Neighbor-Joining phylogenetic analysis the can be divided into three subfamilies-the I/II, IIIA, and IIIB subfamilies, which were unevenly distributed on 13 chromosomes, with the exception of Chr9 and Chr15. Multiple cis-acting regions related to growth and development, as well as stress responses, may be found in the gene promoters. The segmental duplication occurrences greatly aided the evolution of . The results of a collinearity analysis showed that the genes of sweet potato shared evolutionary history with three additional species, including , , and . Additionally, based on the transcriptome sequencing data, the results revealed that the have different expression patterns in leaves, stems, the root body (RB), the distal end (DE), the root stock (RS), the proximal end (PE), the initiative storage root (ISR), and the fibrous root (FR), and many of them are well expressed in the roots. Differentially expressed gene (DEG) analysis of FRs after hormone treatment of the roots indicated that IbXTH28 and IbXTH30 are up-regulated under salicylic acid (SA) treatment but down-regulated under methyl jasmonate (MeJA) treatment. Attentionally, there were only two genes showing down-regulation under the cold and drought treatment. Collectively, all of the findings suggested that genes from the XTH family are crucial for root specificity. This study could provide a theoretical basis for further research on the molecular function of sweet potato genes.
木葡聚糖内转糖基酶/水解酶（XET/XEH，也称为 XTH）家族是一个多基因家族，其功能在植物细胞壁重建和应激耐受中起着重要作用。然而，在甘薯中，尚未对基因家族成员的特定特征及其在不同组织和应激下的表达模式进行研究。在所有的 36 个 XTH 基因中，都具有保守结构（Glyco_hydro_16）。基于邻接法系统发育分析， 可以分为三个亚家族-IA、IIIA 和 IIIB 亚家族，它们不均匀地分布在 13 条染色体上，除了 Chr9 和 Chr15。在 基因启动子中可能存在与生长发育以及应激反应相关的多个顺式作用区域。片段复制的发生极大地促进了 的进化。共线性分析的结果表明，甘薯的 基因与三个附加物种，包括 、 和 ，共享进化历史。此外，基于转录组测序数据，结果表明， 在叶片、茎、薯块（RB）、末端（DE）、薯根（RS）、近端（PE）、初始储存根（ISR）和纤维根（FR）中具有不同的表达模式，其中许多在根部表达良好。对根激素处理后的 FRs 的差异表达基因（DEG）分析表明，IbXTH28 和 IbXTH30 在水杨酸（SA）处理下上调，但在茉莉酸甲酯（MeJA）处理下下调。值得注意的是，只有两个基因在冷和干旱处理下表现出下调。总的来说，所有的发现表明，XTH 家族的基因对根的特异性至关重要。本研究可为进一步研究甘薯 基因的分子功能提供理论基础。
