Yazdi Hamidreza, Seifi Akhtar, Changizi Shima, Khori Vahid, Hossini Fatemeh, Davarian Ali, Jand Yahya, Enayati Ayesheh, Mazandarani Masumeh, Nanvabashi Fateme
Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Department of Botany, Islamic Azad University, Gorgan, Iran.
Avicenna J Phytomed. 2017 Jul-Aug;7(4):334-344.
Several studies have shown the antispasmodic activity of without detailing the underlying mechanism(s). The present study was designed to determine whether the antispasmodic mechanisms of extract mediated via histaminergic/cholinergic receptors, Cachannels, activation of PKA and NO release in isolated rabbit jejunum.
The concentration- dependent (3 × 10-1.3 × 10 mg/ml) antispasmodic effect of the hydro-alcoholic extract of flowers was studied in isolated rabbit jejunum. The isolated jejunum preparations were divided into seven groups, including the pharmacological probes that modulate cholinergic, histaminergic, and nitrergic receptors, as well as PKA.
inhibited spontaneous smooth muscle contractility of the jejunum in a concentration-dependent manner (3 × 10-1.3 × 10 mg/ml) and reduced both K- and Ca-induced contractions, which is similar to the effect of verapamil. The antispasmodic effect of was inhibited by H89 (a PKA inhibitor). The myorelaxant effect of increased in the presence of ACh/His and H89.
evoked antispasmodic and spasmolytic effects mediated through different signaling pathways. Our results have shown this dual inhibitory effect is mediated by blocking Ca channels, activating His and ACh receptors, releasing NO, and activating PKA.
多项研究已表明[具体物质]具有解痉活性，但未详细阐述其潜在机制。本研究旨在确定[具体物质]提取物在离体兔空肠中通过组胺能/胆碱能受体、钙通道、蛋白激酶A（PKA）激活和一氧化氮（NO）释放介导的解痉机制。
研究了[具体物质]花的水醇提取物在离体兔空肠中浓度依赖性（3×10⁻¹.³×10 mg/ml）的解痉作用。将离体空肠标本分为七组，包括调节胆碱能、组胺能和一氧化氮能受体以及PKA的药理学探针。
[具体物质]以浓度依赖性方式（3×10⁻¹.³×10 mg/ml）抑制空肠自发平滑肌收缩，并减少钾离子和钙离子诱导的收缩，这与维拉帕米的作用相似。[具体物质]的解痉作用被H89（一种PKA抑制剂）抑制。在乙酰胆碱/组胺（ACh/His）和H89存在的情况下，[具体物质]的肌松弛作用增强。
[具体物质]通过不同信号通路诱发解痉和解痉作用。我们的结果表明，这种双重抑制作用是通过阻断钙通道、激活组胺和乙酰胆碱受体、释放NO以及激活PKA介导的。
