Departamento Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, Colonia Villa Quietud, México, D.F., México.
Biol Pharm Bull. 2006 Jun;29(6):1186-90. doi: 10.1248/bpb.29.1186.
The antispasmodic activity of extracts from the aerial parts of Buddleja scordioides and Buddleja perfoliata (family: Scrophulariaceae) was studied on isolated tissue preparations from rabbit and guinea pig intestine. The chloroformic extract from the plants exhibited a significant relaxation on the spontaneous contraction of isolated rabbit jejunum at concentrations ranging from 1 to 400 microg/ml, and also caused an inhibitory effect on both K+ and Ca2+ induced contractions in the same tissue. The extracts at moderate doses (50 microg/ml) reduced 5-hydroxytryptamine (5-HT), acetylcholine and histamine induced contractions on isolated guinea pig ileum. Therefore, B. scordioides and B. perfoliata possess similar relaxant mechanism of action, in view of the fact that both inhibit K+ induce contraction and act through serotoninic, muscarinic and histaminic receptors. So, these data support the idea that the extracts may interfere either with calcium mobilization from intracellular stores, or with calcium interaction with regulatory proteins (e.g., calmodulin), or in other steps in the calcium signaling pathway. This leads us to suggest that the spasmolytic effect of both Buddleja species on smooth muscular contractility are due to the same or similar compounds occurring in these two species, which might be present in similar quantities.
研究了玄参科（Scrophulariaceae）植物醉鱼草（Buddleja scordioides）和过路黄（Buddleja perfoliata）地上部分提取物对兔和豚鼠离体肠组织的抗痉挛活性。这两种植物的氯仿提取物在 1 至 400 微克/毫升的浓度范围内对离体兔空肠的自发性收缩表现出显著的松弛作用，同时对同一组织中 K+和 Ca2+诱导的收缩也产生抑制作用。在中等剂量（50 微克/毫升）下，提取物可减轻 5-羟色胺（5-HT）、乙酰胆碱和组胺引起的豚鼠离体回肠收缩。因此，鉴于这两种植物均抑制 K+诱导的收缩并通过 5-羟色胺能、毒蕈碱能和组氨酸能受体起作用，醉鱼草属和过路黄属具有相似的松弛作用机制。因此，这些数据支持这样一种观点，即提取物可能干扰细胞内钙库的钙动员，或者与钙与调节蛋白（如钙调蛋白）的相互作用，或者在钙信号通路的其他步骤中起作用。这使我们推测，这两种醉鱼草属植物对平滑肌收缩的解痉作用是由于这两种植物中存在相同或相似的化合物，而这些化合物可能具有相似的含量。
