Sharma Bhesh Raj, Park Chul Min, Choi Jong Won, Rhyu Dong Young
Department of Oriental Medicine Resources Mokpo National University, Muan-gun, Jeonnam 58554, Republic of Korea.
College of Pharmacy, Kyungsung University, Busan 48434, Republic of Korea.
Avicenna J Phytomed. 2017 Jul-Aug;7(4):366-375.
() Raf. has been used for the prevention and treatment of rheumatoid arthritis, inflammation, and cancer. Our study was designed to unveil the anti-nociceptive and anti-inflammatory effects of the methanolic extract of Raf stem (OHS).
The anti-nociceptive effect was measured by hot plate, acetic acid-induced writhing, and tail flick assays in mice and rats. Moreover, the anti-inflammatory effect was measured by vascular permeability and carrageenan and serotonin-induced paw edema tests in rats. Furthermore, anti-inflammatory effect was also measured using macrophage-like LPS-induced RAW 264.7 cells.
OHS extract inhibited acetic acid-induced writhing (p<0.0001), and delayed the reaction time of mice to the hot plate-induced thermal stimulation (p<0.0001) and tail flick tests (p<0.05). OHS extract attenuated the carrageenan and serotonin-induced paw edema in rats (p<0.001). Similarly, OHS extract significantly decreased Evans blue concentration in acetic acid induced vascular permeability test (p<0.0001), revealing its strong anti-inflammatory effect. Finally, among four different fractions of OHS extract, -butanol fraction strongly decreased NO production (p<0.0001) and iNOS expression in LPS-induced RAW 264.7 cells.
Our results suggest that the methanolic extract of stem can be used to develop a therapeutic or supportive drug and/or functional food against pain and inflammation related diseases.
（）Raf. 已被用于预防和治疗类风湿性关节炎、炎症和癌症。我们的研究旨在揭示 Raf 茎甲醇提取物（OHS）的抗伤害感受和抗炎作用。
通过热板法、乙酸诱导扭体法和小鼠及大鼠甩尾试验测定抗伤害感受作用。此外，通过大鼠血管通透性、角叉菜胶和血清素诱导的爪肿胀试验测定抗炎作用。此外，还使用巨噬细胞样 LPS 诱导的 RAW 264.7 细胞测定抗炎作用。
OHS 提取物抑制乙酸诱导的扭体反应（p<0.0001），并延迟小鼠对热板诱导的热刺激的反应时间（p<0.0001）和甩尾试验的反应时间（p<0.05）。OHS 提取物减轻了大鼠角叉菜胶和血清素诱导的爪肿胀（p<0.001）。同样，OHS 提取物在乙酸诱导的血管通透性试验中显著降低伊文思蓝浓度（p<0.0001），显示出其强大的抗炎作用。最后，在 OHS 提取物的四个不同组分中，正丁醇组分强烈降低 LPS 诱导的 RAW 264.7 细胞中 NO 的产生（p<0.0001）和 iNOS 的表达。
我们的结果表明，Raf 茎甲醇提取物可用于开发针对疼痛和炎症相关疾病的治疗或辅助药物和/或功能性食品。
