Tekulu Gebretsadkan Hintsa, Desta Abrha, Hiben Mebrahtom Gebrelibanos, Araya Ephrem Mebrahtu
Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia.
Department of Pharmacy, College of Medicine and Health Sciences, Adigrat University, Adigrat, Ethiopia.
J Inflamm Res. 2020 Sep 4;13:497-505. doi: 10.2147/JIR.S269717. eCollection 2020.
The management of pain and inflammation with non-steroidal anti-inflammatory drugs and opioid analgesics are currently encountering severe adverse reactions. To overcome these problems, herbal remedies may offer new alternative medicines. is a medicinal plant traditionally used for the treatment of pain and inflammation-related disorders; yet, these claims are not scientifically validated. Hence, this study was aimed to validate the traditional use of leaves as anti-inflammatory and analgesic remedy.
In vitro anti-hyaluronidase assay and in vivo carrageenan-induced hind paw oedema model were used to evaluate the anti-inflammatory property of ethanolic leaf extract of . Tail immersion and acetic acid-induced writhing tests were performed to determine the central and peripheral analgesic activity of the leaf extract, respectively.
The ethanolic leaf extract exhibited significant anti-hyaluronidase activity (<0.001) and significant inhibition of carrageenan-induced paw oedema (<0.05) compared to untreated controls. Similarly, the extract significantly prolonged the reaction time of mice (<0.05) for the hot-water stimuli. Furthermore, an oral dose of the extract showed significant inhibition (<0.01) of acetic acid-induced abdominal contractions of mice. Besides, the ethanolic leaf extract did not cause any obvious sign of acute toxicity at a single oral dose of 2 g/kg.
The findings of this study may partially support the acclaimed traditional use of leaves for the treatment of pain and inflammatory conditions.
目前，使用非甾体抗炎药和阿片类镇痛药来管理疼痛和炎症正面临严重的不良反应。为克服这些问题，草药疗法可能提供新的替代药物。[植物名称]是一种传统上用于治疗与疼痛和炎症相关疾病的药用植物；然而，这些说法尚未得到科学验证。因此，本研究旨在验证[植物名称]叶作为抗炎和镇痛药物的传统用途。
采用体外抗透明质酸酶测定法和体内角叉菜胶诱导的后爪水肿模型来评估[植物名称]乙醇叶提取物的抗炎特性。分别进行尾部浸入试验和乙酸诱导的扭体试验，以确定叶提取物的中枢和外周镇痛活性。
与未处理的对照组相比，乙醇叶提取物表现出显著的抗透明质酸酶活性（<0.001）和对角叉菜胶诱导的爪水肿的显著抑制作用（<0.05）。同样，该提取物显著延长了小鼠对热水刺激的反应时间（<0.05）。此外，口服该提取物对乙酸诱导的小鼠腹部收缩有显著抑制作用（<0.01）。此外，乙醇叶提取物在单次口服剂量为2 g/kg时未引起任何明显的急性毒性迹象。
本研究结果可能部分支持[植物名称]叶用于治疗疼痛和炎症性疾病的传统说法。
