Kollias G, Evans D J, Ritter M, Beech J, Morris R, Grosveld F
Laboratory of Gene Structure and Expression, National Institute for Medical Research, Ridgeway, Mill Hill, London, England.
Cell. 1987 Oct 9;51(1):21-31. doi: 10.1016/0092-8674(87)90006-7.
Hybrid human--mouse Thy-1.1 genes were injected into pronuclei of Thy-1.2 mice to produce transgenic animals. A hybrid gene composed of the 5' part of the mouse Thy-1.1 gene combined with the 3' human untranslated regions was expressed abnormally in the kidney podocytes, which resulted in severe protein-uria and subsequent death in several founder mice. A hybrid Thy-1 gene composed of the human coding region with the 5' and 3' flanking regions of the mouse gene was expressed abnormally in a different part of the kidney (the tubular epithelia), which resulted in a proliferative kidney disorder. In addition, a neoplasm was found in the brain of one of these mice. These results show that the Thy-1 protein can play an important role in the activation, proliferation, and differentiation of many different cell types.
将人鼠杂交的Thy-1.1基因注射到Thy-1.2小鼠的原核中以产生转基因动物。由小鼠Thy-1.1基因的5'部分与3'人非翻译区组成的杂交基因在肾足细胞中异常表达，导致几只奠基小鼠出现严重蛋白尿并随后死亡。由人编码区与小鼠基因的5'和3'侧翼区组成的杂交Thy-1基因在肾脏的不同部位（肾小管上皮）异常表达，导致肾脏增生性疾病。此外，在其中一只小鼠的大脑中发现了肿瘤。这些结果表明，Thy-1蛋白在许多不同细胞类型的激活、增殖和分化中可能发挥重要作用。
