Gordon J W, Chesa P G, Nishimura H, Rettig W J, Maccari J E, Endo T, Seravalli E, Seki T, Silver J
Cell. 1987 Jul 31;50(3):445-52. doi: 10.1016/0092-8674(87)90498-3.
Genomic DNA fragments encompassing the human Thy-1 or mouse Thy-1.1 gene have been microinjected into pronuclei of mouse embryos homozygous for the Thy-1.2 allele. In the resulting transgenic mice, the human gene is expressed in a pattern characteristic of normal human tissues, and is not influenced by the pattern of endogenous mouse Thy-1 expression. The mouse Thy-1.1 gene fragment is expressed in a pattern typical of mouse Thy-1, although it is more limited in its distribution. The results indicate the presence of multiple cis-acting regulators of Thy-1 gene expression that have changed in both their character and arrangement over the course of Thy-1 gene evolution.
包含人类Thy-1或小鼠Thy-1.1基因的基因组DNA片段已被显微注射到Thy-1.2等位基因纯合的小鼠胚胎原核中。在产生的转基因小鼠中，人类基因以正常人类组织特有的模式表达，且不受内源性小鼠Thy-1表达模式的影响。小鼠Thy-1.1基因片段以小鼠Thy-1典型的模式表达，尽管其分布更有限。结果表明存在Thy-1基因表达的多个顺式作用调节因子，它们在Thy-1基因进化过程中其性质和排列都发生了变化。
