Electrochemical nitric oxide biosensor based on amine-modified MoS/graphene oxide/myoglobin hybrid.

Nitric oxide (NO) is one of the most important molecules in living things due to its role as a signaling molecule in influencing pathological and physiological mechanisms including neurotransmission. In this study, the electrochemical biosensor based on the amine-modified molybdenum disulfide nanoparticles (MoS), graphene oxide (GO) and myoglobin (Mb) hybrid material (amine-modified MoS/GO/Mb hybrid) is developed to achieve the accurate detection of NO with electrochemical signal improvement. For the first time, the synthesis of MoS accompanying the amine-modification of the surface of MoS is done to hybridize with GO efficiently through the short linkage. After the amine-modification of MoS, it is enclosed with GO directly (amine-modified MoS/GO). Then, Mb which can induce the reduction of NO is immobilized on the amine-modified MoS/GO to fabricate the amine-modified MoS/GO/Mb hybrid for NO detection. The prepared hybrid shows the signal improved redox properties relative to the result of the electrode prepared without hybrid. Furthermore, upon addition of NO, the electrode prepared with hybrid shows the improved amperometric response compared with that of the electrode without hybrid. This amine-modified MoS/GO/Mb hybrid can be used in the development of the biosensor platform accompanying the electrochemical signal improvement and accurate detection of target materials.