金黄色葡萄球菌中NorA多药外排泵的优化外排测定法。
Optimized efflux assay for the NorA multidrug efflux pump in Staphylococcus aureus.
作者信息
Zimmermann Saskia, Tuchscherr Lorena, Rödel Jürgen, Löffler Bettina, Bohnert Jürgen A
机构信息
Institute of Medical Microbiology, Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany; Jena University Hospital, Integrated Research and Treatment Center, Center for Sepsis Control and Care (CSCC), Am Klinikum 1, D-07747 Jena, Germany.
Institute of Medical Microbiology, Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany; Jena University Hospital, Integrated Research and Treatment Center, Center for Sepsis Control and Care (CSCC), Am Klinikum 1, D-07747 Jena, Germany.
出版信息
J Microbiol Methods. 2017 Nov;142:39-40. doi: 10.1016/j.mimet.2017.09.001. Epub 2017 Sep 5.
Real-time fluorescent efflux assays are commonly used for measuring the efflux of bacterial pumps. Here we describe an optimized protocol for the NorA efflux pump in S. aureus using DiOC instead of ethidium bromide. Glucose and sodium formate were tested as energy carriers. This novel method is fast and reproducible.
实时荧光外排测定法通常用于测量细菌泵的外排。在此，我们描述了一种使用DiOC而非溴化乙锭来优化金黄色葡萄球菌中NorA外排泵的实验方案。测试了葡萄糖和甲酸钠作为能量载体。这种新方法快速且可重复。
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