Wang Kunyang, Lin Zhishan, Wang Long, Wang Ke, Shi Qinghua, Du Lipu, Ye Xingguo
National Key Facility of Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
Theor Appl Genet. 2018 Jan;131(1):13-25. doi: 10.1007/s00122-017-2982-5. Epub 2017 Sep 8.
Transcriptome data were used to develop 134 Aegilops longissima specific PCR markers and their comparative maps were constructed by contrasting with the homologous genes in the wheat B genome. Three wheat- Ae. longissima 1BL·1S S translocation lines were identified using the correspondence markers. Aegilops longissima is an important wild species of common wheat that harbors many genes that can be used to improve various traits of common wheat (Triticum aestivum L.). To efficiently transfer the traits conferred by these Ae. longissima genes into wheat, we sequenced the whole expression transcript of Ae. longissima. Using the transcriptome data, we developed 134 specific polymerase chain reaction markers located on the 14 chromosome arms of Ae. longissima. These novel molecular markers were assigned to specific chromosome locations based on a comparison with the homologous genes in the B genome of wheat. Annotation of these genes showed that most had functions related to metabolic processes, hydrolase activity, or catalytic activity. Additionally, we used these markers to identify three wheat-Ae. longissima 1BL·1SS translocation lines in somatic variation populations resulting from a cross between wheat cultivar Westonia and a wheat-Ae. longissima substitution line 1S(1B). The translocation lines had several low molecular weight glutenin subunits encoding genes beneficial to flour processing quality that came from Ae. longissima 1SS. The three translocation lines were also confirmed by genomic in situ hybridization. These translocation lines will be further evaluated for potential quality improvement of bread-making properties of wheat.
转录组数据被用于开发134个节节麦特异性PCR标记，并通过与小麦B基因组中的同源基因对比构建其比较图谱。利用对应标记鉴定出了三个小麦-节节麦1BL·1S易位系。节节麦是普通小麦的一个重要野生种，含有许多可用于改良普通小麦（Triticum aestivum L.）各种性状的基因。为了有效地将这些节节麦基因赋予的性状转移到小麦中，我们对节节麦的整个表达转录本进行了测序。利用转录组数据，我们开发了134个位于节节麦14条染色体臂上的特异性聚合酶链反应标记。通过与小麦B基因组中的同源基因比较，将这些新的分子标记定位到特定的染色体位置。对这些基因的注释表明，大多数基因具有与代谢过程、水解酶活性或催化活性相关的功能。此外，我们利用这些标记在小麦品种韦斯托尼亚与小麦-节节麦代换系1S(1B)杂交产生的体细胞变异群体中鉴定出三个小麦-节节麦1BL·1SS易位系。这些易位系含有几个对面粉加工品质有益的低分子量谷蛋白亚基编码基因，这些基因来自节节麦1SS。这三个易位系也通过基因组原位杂交得到了证实。这些易位系将进一步评估其对小麦面包制作特性潜在的品质改良作用。
