College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.
Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD 57007 U.S.A.
Plant Dis. 2021 Oct;105(10):2938-2945. doi: 10.1094/PDIS-12-20-2691-RE. Epub 2021 Oct 26.
Powdery mildew of wheat, caused by f. sp. , is a destructive disease of common wheat. Cultivation of resistant varieties is the most cost-effective disease management strategy. Previous studies reported that chromosome 3S#2 present in Chinese Spring (CS)- 3S#2(3B) disomic substitution line TA3575 conferred resistance to powdery mildew. In this study, we further located the powdery mildew resistance gene(s) to the short arm of chromosome 3S#2 (3S#2S) by evaluating for f. sp. resistance of newly developed CS- 3S#2 translocation lines. Meanwhile, TA7545, a previously designated CS- 3S#3 disomic addition line, was reidentified as an isochromosome 3S#3S addition line and evaluated to confer resistance to powdery mildew, thus locating the resistance gene(s) to the short arm of chromosome 3S#3 (3S#3S). Based on transcriptome sequences of TA3575, 10 novel chromosome 3SS-specific markers were developed, of which 5 could be used to distinguish between 3S#2S and 3S#3S derived from accessions TL20 and TA1910 (TAM4) and the remaining 5 could identify both 3S#2S and 3S#3S. Also, CL897, one of five markers specific to both 3S#2S and 3S#3S, could be used to detect located at chromosome 3S#1S from accession TL01 in diverse wheat genetic backgrounds. The powdery mildew resistance genes on chromosomes 3S#2S and 3S#3S, the CS- 3S#2 translocation lines, and the 3SS-specific markers developed in this study will facilitate the transfer of f. sp. resistance genes into common wheat and provide new germplasm resources for powdery mildew resistance breeding.
小麦白粉病由 f. sp. 引起，是普通小麦的一种破坏性疾病。种植抗性品种是最具成本效益的疾病管理策略。先前的研究报告称，中国春（CS）- 3S#2（3B）二体异附加系 TA3575 中存在的 3S#2 染色体上的 3S#2S 赋予了对白粉病的抗性。在本研究中，我们通过评估新开发的 CS- 3S#2 易位系对 f. sp. 的抗性，进一步将白粉病抗性基因（s）定位到 3S#2S 短臂上。同时，重新鉴定先前指定的 CS- 3S#3 二体附加系 TA7545 为 3S#3S 等臂染色体附加系，并对其抗白粉病进行评估，从而将抗性基因（s）定位到 3S#3S 短臂上。基于 TA3575 的转录组序列，开发了 10 个新的 3SS 特异性标记，其中 5 个可用于区分 TL20 和 TA1910（TAM4）品系来源的 3S#2S 和 3S#3S，其余 5 个可同时鉴定 3S#2S 和 3S#3S。此外，5 个 3S#2S 和 3S#3S 共有的标记之一 CL897，也可用于检测 TL01 品系来源的染色体 3S#1S 上的 3S#1S 上的 3S#1S。本研究中开发的 3S#2S 和 3S#3S 染色体上的白粉病抗性基因、CS- 3S#2 易位系和 3SS 特异性标记将有助于将 f. sp. 抗性基因转移到普通小麦中，并为白粉病抗性育种提供新的种质资源。
