Department of Studies and Research in Chemistry, UCS, Tumkur University, Tumkur 572103, Karnataka, India.
Department of Biotechonology, UCS, Tumkur University, Tumkur 572103, Karnataka, India.
Mater Sci Eng C Mater Biol Appl. 2017 Dec 1;81:182-190. doi: 10.1016/j.msec.2017.07.054. Epub 2017 Aug 1.
The current work portrays the green synthesis of Lignin Capped Silver Nanoparticles (LCSN) and their antibacterial, antifungal, antioxidant and antiplatelet potential. The LCSN was synthesized in water using a carbohydrate based polymer 'lignin' as the reducing and capping agents. The peak at 406nm (λ) in the UV-Vis., spectrum and EDX analysis confirmed 1.68% (w/w) of silver was found to be loaded on lignin. The characteristic sharp peaks appeared in the PXRD spectrum showed fcc crystalline structure LCSN. SEM and TEM images indicated that the spherical Ag-NPs were well dispersed on lignin with an average particle size of ~10-15nm. LCSN showed antibacterial and antifungal activity against human pathogens S. aureus, E. coli and A. niger and the percentage of zone of inhibition was found to be 10%, 12% and 80% respectively. Further, LCSN was evaluated for antioxidant potential using DPPH scavenging assay, interestingly it showed antioxidant activity and the percentage against positive control vitamin C was found to be 70%. Furthermore, LCSN did not interfere in plasma coagulation; however, it found to inhibit agonist ADP induced platelet aggregation of human platelet rich plasma. The observed inhibition was found to be 37% and the calculated IC50 value was found to be 9mg/mL. LCSN did not lyses RBC membrane when assayed hemolytic activity suggesting its non-toxic nature.
本研究描述了木质素包覆银纳米粒子（LCSN）的绿色合成及其抗菌、抗真菌、抗氧化和抗血小板作用。采用碳水化合物基聚合物“木质素”作为还原剂和稳定剂，在水中合成 LCSN。紫外-可见光谱（UV-Vis.）的 406nm 处的峰值和 EDX 分析证实，木质素上负载了 1.68%（w/w）的银。X 射线粉末衍射（PXRD）图谱中出现的特征尖锐峰表明 LCSN 具有面心立方（fcc）结晶结构。SEM 和 TEM 图像表明，球形 Ag-NPs 很好地分散在木质素上，平均粒径约为 10-15nm。LCSN 对人类病原体金黄色葡萄球菌、大肠杆菌和黑曲霉表现出抗菌和抗真菌活性，抑菌圈直径分别为 10%、12%和 80%。此外，LCSN 的抗氧化活性采用 DPPH 清除实验进行评价，其具有抗氧化活性，与阳性对照维生素 C 的抑制率为 70%。此外，LCSN 不会干扰血浆凝固，但发现它可以抑制人富含血小板血浆中激动剂 ADP 诱导的血小板聚集。观察到的抑制率为 37%，计算得出的 IC50 值为 9mg/mL。当测定溶血活性时，LCSN 不会裂解 RBC 膜，表明其无毒性质。
