Petrova Olga E, Sauer Karin
Department of Biological Sciences, Binghamton Biofilm Research Center (BBRC), Binghamton University, 2401 ITC Building, 85 Murray Hill Road, Binghamton, NY, 13902, USA.
Methods Mol Biol. 2017;1657:33-43. doi: 10.1007/978-1-4939-7240-1_4.
The modulation of c-di-GMP levels plays a vital role in the regulation of various processes in a wide array of bacterial species. Thus, investigation of c-di-GMP regulation requires reliable methods for the assessment of c-di-GMP levels and turnover. Reversed-phase high-performance liquid chromatography (RP-HPLC) analysis has become a commonly used approach to accomplish these goals. The following describes the extraction and HPLC-based detection and quantification of c-di-GMP from Pseudomonas aeruginosa samples, a procedure that is amenable to modifications for the analysis of c-di-GMP in other bacterial species.
环二鸟苷酸（c-di-GMP）水平的调节在众多细菌物种的各种生理过程调控中起着至关重要的作用。因此，研究c-di-GMP的调节需要可靠的方法来评估其水平和周转情况。反相高效液相色谱（RP-HPLC）分析已成为实现这些目标的常用方法。以下介绍了从铜绿假单胞菌样品中提取c-di-GMP并基于HPLC进行检测和定量的方法，该方法可经修改用于分析其他细菌物种中的c-di-GMP。
