a Antioxidants Research Laboratory , Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University , Boston , MA , USA.
b Guangzhou University of Chinese Medicine , Guangzhou , Guangdong , PR China.
Int J Food Sci Nutr. 2018 May;69(3):377-383. doi: 10.1080/09637486.2017.1371116. Epub 2017 Sep 11.
Oat avenanthramides (AVAs) are a group of phenolic alkaloids, consisting of an anthranilic acid and a hydroxycinnamic acid linked by a pseudo-peptide bond. Bioavailability of AVA is poor in humans, suggesting transformations for rapid excretion. Thus, we aim to identify metabolites of AVA isomers in plasma of humans after consuming AVA-enriched oats. After lipid removal, AVA and their metabolites in plasma were extracted with ethyl acetate and analysed using an Agilent UHPLC-QToF-MS. Pharmacokinetics of AVA-O showed a bimodal distribution with C1 and 2 for AVA-O at 5.9 ± 5.2 and 7.9 ± 7.0 ng/mL and T1 and 2 at 1.7 ± 0.7 and 3.1 ± 1.2 h, respectively. Only the methyl-AVA-O showed a single C at 14 ± 9.9 ng/mL AVA-O equivalents and a T of 2.4 ± 2.7 h. This analysis is the first to identify methylated metabolites of AVAs and AVA aglycones in human blood after acute AVA consumption.
燕麦蒽酰胺（AVAs）是一组酚类生物碱，由邻氨基苯甲酸和羟基肉桂酸通过拟肽键连接而成。AVAs 在人体内的生物利用度较差，表明其会迅速转化为代谢物而排泄。因此，我们旨在鉴定人类食用富含 AVAs 的燕麦后血浆中 AVAs 异构体的代谢物。在去除脂质后，用乙酸乙酯提取血浆中的 AVAs 和它们的代谢物，并使用安捷伦 UHPLC-QToF-MS 进行分析。AVA-O 的药代动力学呈双峰分布，C1 和 C2 分别为 5.9±5.2 和 7.9±7.0ng/mL，T1 和 T2 分别为 1.7±0.7 和 3.1±1.2h。只有甲基-AVA-O 显示出单一的 C，其浓度为 14±9.9ng/mL AVA-O 当量，T 为 2.4±2.7h。这项分析首次鉴定了急性 AVA 摄入后人类血液中 AVAs 和 AVA 苷元的甲基化代谢物。
