Pooja Shetty, Sabeena Sasidharanpillai, Revti Bhaskar, Sanjay Ramachandran, Anjali Aithal, Rajendra Kumar, Aswathyraj Sushama, Giselle Dsouza, Hindol Maity, Arunkumar Govindakarnavar
Manipal Centre for Virus Research, Manipal University.
Jpn J Infect Dis. 2017 Nov 22;70(6):663-665. doi: 10.7883/yoken.JJID.2017.087. Epub 2017 Sep 11.
Dengue is the most prevalent arboviral disease affecting humans, with tropical and subtropical regions having the highest risk of dengue virus infections. The clinical symptoms often range from mild fever to fever with hemorrhagic manifestations and shock. Since 2010, India has been witnessing a marked increase in the number of dengue virus serotype 1 (DENV-1) infections, apart from those of serotypes 2 (DENV-2) and 3 (DENV-3). The present study was undertaken to understand the circulating genotypes of DENV-1 in South West India, by sequencing the envelope gene of DENV-1 samples representative of the 2014-2015 time period. The extracted RNAs from dengue NS1 antigen ELISA-positive samples, archived at the APEX Referral Laboratory for Arboviruses (National Vector Borne Disease Control Programme, Manipal Centre for Virus Research), were used for the study. The currently circulating genotypes of DENV-1 identified from the study area belong to genotypes V and I. Multicentric laboratory-based studies analyzing the envelope gene of all dengue serotypes are required from endemic countries like India.
登革热是影响人类的最普遍的虫媒病毒疾病，热带和亚热带地区登革热病毒感染风险最高。临床症状通常从轻度发热到伴有出血表现和休克的发热。自2010年以来，除了2型登革热病毒（DENV-2）和3型登革热病毒（DENV-3）感染外，印度1型登革热病毒（DENV-1）感染数量显著增加。本研究旨在通过对代表2014 - 2015年期间的DENV-1样本的包膜基因进行测序，了解印度西南部DENV-1的流行基因型。从登革热NS1抗原ELISA阳性样本中提取的RNA，保存在APEX虫媒病毒转诊实验室（国家媒介传播疾病控制计划，马尼帕尔病毒研究中心），用于本研究。从研究区域鉴定出的目前流行的DENV-1基因型属于V型和I型。像印度这样的流行国家需要开展基于多中心实验室的研究，分析所有登革热血清型的包膜基因。
