Zhou Wenhao, Rahimnejad Samad, Lu Kangle, Wang Lina, Liu Wenbin
Fisheries College, Jimei University, Xiamen, 361021, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Fish Physiol Biochem. 2019 Feb;45(1):83-91. doi: 10.1007/s10695-018-0536-7. Epub 2018 Jul 8.
Fatty liver of cultured fish often correlates closely with poor growth and low harvest yield. Some Chinese herbs can reduce hepatic fat storage. This study aimed to examine lipid-lowering effect of berberine (BBR) in blunt snout bream (Megalobrama amblycephala). Triplicate groups of fish were fed four experimental diets: low-fat diet (LFD, 5% fat), high-fat diet (HFD, 15% fat), and HFD supplemented with 50 or 100 mg BBR/kg diet (BBR50, BBR100). After 8-week feeding, growth performance, liver histology and fat deposition, and hepatic genes expression were examined. The results showed significant reduction of growth performance and feed intake in fish fed HFD compared to those fed the LFD and BBR50 diets. Supplementing 50 mg BBR/kg to the HFD significantly improved weight gain and feed intake. Higher hepatic fat content and histological abnormalities were found in the liver of fish receiving HFD, and BBR50 and BBR100 could attenuate these abnormalities of liver. Expression of CPT I, AOX, ApoB100, ApoE, and PGC-1α genes was significantly decreased in fish fed HFD, and 50 and 100 mg/kg BBR supplementation could revert the downregulation of these genes. Also, the expression of FATP, LPL, and LDLR genes was upregulated in HFD-fed fish, and their expression was significantly decreased by 50 and 100 mg/kg BBR supplementation. In conclusion, supplementing BBR to HFD could attenuate liver fat deposition and disorders. The fat-lowering effects of BBR appear to be mediated by activating genes related with fatty acid oxidation and decreasing genes for fatty acid uptake.
养殖鱼类的脂肪肝通常与生长缓慢和低收获产量密切相关。一些中草药可以减少肝脏脂肪储存。本研究旨在检测黄连素（BBR）对团头鲂（Megalobrama amblycephala）的降脂作用。将鱼分成三组，分别投喂四种实验饲料：低脂饲料（LFD，5%脂肪）、高脂饲料（HFD，15%脂肪）以及添加了50或100 mg BBR/kg饲料的高脂饲料（BBR50、BBR100）。经过8周的投喂后，检测生长性能、肝脏组织学和脂肪沉积以及肝脏基因表达。结果显示，与投喂LFD和BBR50饲料的鱼相比，投喂HFD的鱼生长性能和采食量显著降低。在HFD中添加50 mg BBR/kg可显著提高体重增加和采食量。在接受HFD的鱼的肝脏中发现了更高的肝脏脂肪含量和组织学异常，而BBR50和BBR100可以减轻这些肝脏异常。在投喂HFD的鱼中，CPT I、AOX、ApoB100、ApoE和PGC-1α基因的表达显著降低，添加50和100 mg/kg BBR可使这些基因的下调恢复正常。此外，在投喂HFD的鱼中，FATP、LPL和LDLR基因的表达上调，添加50和100 mg/kg BBR可使其表达显著降低。总之，在HFD中添加BBR可以减轻肝脏脂肪沉积和紊乱。BBR的降脂作用似乎是通过激活与脂肪酸氧化相关的基因并减少脂肪酸摄取相关基因来实现的。
