Zhu Bo, Liu Jing-Jing, Si Jin-Ping, Qin Lu-Ping, Han Ting, Zhao Li, Wu Ling-Shang
Zhejiang Chinese Medical University, School of Pharmacy, Hangzhou 310053, China.
Engineering and Technical Research Center of Dendrobium officinale of State Forestry Administration, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
Zhongguo Zhong Yao Za Zhi. 2016 May;41(9):1602-1607. doi: 10.4268/cjcmm20160906.
The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings. Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured. Polysaccharides and alcohol-soluble extracts contents were determined by phenol-sulfuric acid method and hot-dipmethod, respectively. Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively. It showed that effects of turning to purple of stem nodes could be changed by endophytic fungus. Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts. The strains tested, expect DO34, could promote growth and polysaccharides content of tissue culture seedlings. The strains tested, expect DO12, could promote the accumulation of mannose. Furthermore, DO18, DO19 and DO120 could increase alcohol-soluble extracts. On the basis, four superior strains were selected for mechanism research between endophytic fungus and their hosts and microbiology engineering.
本文旨在研究活树上栽培的铁皮石斛内生真菌对组培苗生长及成分代谢的影响。观察并测定了感染内生真菌和未感染内生真菌的组培苗的形态特征和农艺性状。分别采用苯酚-硫酸法和热浸法测定多糖和醇溶性提取物的含量。分别采用柱前衍生高效液相色谱法和高效液相色谱法分析多糖的单糖组成和醇溶性提取物的成分。结果表明，内生真菌可改变茎节变紫的效果。此外，内生真菌可影响多糖和醇溶性提取物的含量和组成。除DO34外，所测菌株均可促进组培苗的生长和多糖含量。除DO12外，所测菌株均可促进甘露糖的积累。此外，DO18、DO19和DO120可增加醇溶性提取物。在此基础上，筛选出4株优良菌株，用于内生真菌与宿主之间的作用机制研究及微生物工程研究。
