Institut Européen des Membranes, UMR 5635, Université Montpellier, CNRS, ENSCM , Place Eugene Bataillon, Montpellier Cedex 5 F-34095, France.
Crystal Growth Centre, Anna University , Chennai 600025, India.
ACS Appl Mater Interfaces. 2017 Oct 4;9(39):33695-33706. doi: 10.1021/acsami.7b13199. Epub 2017 Sep 21.
Gelatin is a biodegradable biopolymer obtained by collagen denaturation, which shows poor mechanical properties. Hence, improving its mechanical properties is very essential toward the fabrication of efficient nontoxic material for biomedical applications. For this aim, various methods are employed using external fillers such as ceramics or bioglass. In this report, we introduce boron nitride (BN)-reinforced gelatin as a new class of two-dimensional biocompatible nanomaterials. The effect of the nanofiller on the mechanical behavior is analyzed. BN is efficiently exfoliated using the biopolymer gelatin as shown through Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). The exfoliated BN reinforces gelatin electrospun fibers, which results in an increase in the Young's modulus. The Electrospun Mats (ESM) are stable after the glutaraldehyde cross-linking, and the fibrous morphology is preserved. The cross-linked gelatin/BN ESM is highly bioactive in forming bonelike hydroxyapatite as shown by scanning electron microscopy. Due to their enhanced mineralization ability, the cross-linked ESM have been tested on human bone cells (HOS osteosarcoma cell line). The cell attachment, proliferation, and biocompatibility results show that the ESM are nontoxic and biodegradable. The analysis of osteoblast gene expression and the measurement of alkaline phosphatase activity confirm that these materials are suitable for bone tissue engineering.
明胶是一种通过胶原蛋白变性获得的可生物降解的生物聚合物，其机械性能较差。因此，提高其机械性能对于制造用于生物医学应用的高效无毒材料非常重要。为此，采用了各种方法，使用外部填充剂（如陶瓷或生物玻璃）。在本报告中，我们介绍了氮化硼（BN）增强明胶作为一种新型二维生物相容性纳米材料。分析了纳米填料对机械性能的影响。通过傅里叶变换红外（FTIR）光谱和 X 射线衍射（XRD）显示，BN 可有效地剥离生物聚合物明胶。剥离的 BN 增强了明胶静电纺纤维，从而提高了杨氏模量。经戊二醛交联后，静电纺丝毡（ESM）稳定，保留了纤维形态。扫描电子显微镜显示，交联明胶/BN ESM 具有高度的成骨活性，可形成类骨羟基磷灰石。由于其增强的矿化能力，交联 ESM 已在人骨肉瘤细胞系（HOS）上进行了测试。细胞附着、增殖和生物相容性结果表明，这些材料是无毒和可生物降解的。成骨细胞基因表达分析和碱性磷酸酶活性的测量证实，这些材料适合骨组织工程。
